LOCUS       sequence-89859-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HPDA-c103
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(98..121)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(122..139)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(140..160)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atcctcactt atcgtcgtca tccttgtaat
      121 catggtggtg atgatggtgc gattggaagt agaggttctc tgccatgccg ggcaccaccc
      181 cattggtctc catgttggtg aggttacccc gcaggttctg ctcctcctcg aaagccttga
      241 acagtgagtt gaagttgccg gctccaaaac cctggtggtt gtggcgctgg atgacttcca
      301 ggaagagcgt gggccggtcc tgcaccggtt tggtgaagat ctgcaggagg tagcctttct
      361 cgtcgtagtc caccaggatt ttcagctcct ccagggcatc aatgttctcc ttcaccttga
      421 tcttggccgt cttcagcttc tcccgcagtt gtttgtagta cgtggaggga acagataaga
      481 actccaggcc tctctctctc aagtggcgaa tcgctgtgat gatgtcttcg gtcttgagag
      541 cgatgtgctg gaccccagcg cccccgttat agtccacata ttcctggatc tgggacttct
      601 tcttgccagg cgctggctca ttgatgggca tcttgatgga ctcttcatag ttggccacca
      661 caatggatcg cagagagcta tattccgtgt gcacctgcgt gtcatccacg gaccagaagc
      721 ggtggaactg caggtttttc aggtaccatt cggaggcgga caccatctcc tgatcaggct
      781 ggtttcccac aatgtggtcg atcatctcca gactgcattt gggcagttta ggaagtaggg
      841 ggtccatgaa cgctggggcc tcatatccag gcaagaattg gccgatgtag ttcatcttct
      901 ccaccagggt gtgtgtggtg tccccatacg tctgcagcac agcaaacttc accttcccaa
      961 acttgtcttg ctctacc
//
