LOCUS       sequence-89863-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ELAC1A-c005
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(96..119)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(120..137)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(138..158)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcggat cctcacttat cgtcgtcatc cttgtaatca
      121 tggtggtgat gatggtgcga ttggaagtag aggttctctg ctttcttgat tggaatgctt
      181 atcaccataa aatcttctgc tagagtcact tcttggagat ctaacactga ttcagcttgc
      241 ttttttagtt ctgcaatgcc atctgtttct ccttctctgg ccaaggcaac tggtttgtac
      301 ctctgactga agtgagtcag aaccagcctc tttgcacggc acaactttgc aaatgttgct
      361 gccatctgtg gtgtgctgtg gccatgctcc tttgctttgt ccatctgggc atcatccagg
      421 gttgcttcgt ggatcaacag gtctgcttca aagcacagtt ttactcctcc atcacccaca
      481 accccagagc agtcacccaa tatgcagatt tttcttccaa caataggctt ttttaagaca
      541 tcttggggag aaattgtaac cccattttcc agaacaacag aaattccatt tttcagcttc
      601 ccataggcag gacctggtgg aacaccaagg tctttaagtt tctgtgcatt gagtttacct
      661 gggcgtttct tttccacgac tgaaaaccca aatgagggaa ttctgtgaaa gaggcgaaat
      721 gcttttacaa caaattgttc atcatc
//
