LOCUS       sequence-89866-e       11501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  const. Mis12-FKBP3; ind. FRB-mCH-Mad1^485-718.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11501)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 11501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB171
FEATURES             Location/Qualifiers
     source          1..11501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    52..100
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    312..345
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     ncRNA           501..527
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           591..632
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     CDS             1410..1730
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1737..2057
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1737..2057
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             2070..2393
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     misc_feature    join(2454..3028,3029..3031,3032..3040)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2621..2638)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2848..2867
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3041..3640
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3041..3060)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3537..3557
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(3755..3772)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3761..3872
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3968..4089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4221..4928)
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     promoter        complement(4939..6150)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(5083..5102)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    6172..6190
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6207..6225
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6243..6261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6279..6297
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6314..6332
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6350..6368
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6380..6418
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6415..6439
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     ncRNA           6596..6622
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           6648..6689
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     CDS             6830..7108
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     regulatory      7121..7130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7127..7834
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7272..7290)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7540..7559
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8648..8667)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8713..8768
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8767..8786)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    9129..9162
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    9175..9296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9315..9335)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9315..9333)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9354..9370)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9354..9370)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9367..9389)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9378..9394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9402..9408,9409..9426,9427..9432))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9447..9468
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9585..9602)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9756..10344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9836..9855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10515..11306,11307..11375))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11138..11157
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11376..11480)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagcttaata aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
       61 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
      121 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
      181 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
      241 gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca
      301 ttcgagaata aataacttcg tataaagtat cctatacgaa gttatcaccc tgcagtacgt
      361 atccggtagc cttgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca
      421 agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt
      481 ttcggtcgac ttctagagct ctggaggctt gctgaaggct gtatgctgat atggattcat
      541 gtgcacatgg ttttggccac tgactgacca tgtgcatgaa tccatatcag gacacaaggc
      601 ctgttactag cactcacatg gaacaaatgg ccgaattcgg cgctagctgc tgatatcgca
      661 tacgcgtgga ccagataggc acctattggt cttactgaca tccactttgc ctttctctcc
      721 acaggtgtcg gtaccgcggg cccgggatcc accggcgcgc caccatgtct gtggatccaa
      781 tgacctacga ggcccagttc tttggcttca cgccacaaac gtgcatgctt cggatctaca
      841 ttgcatttca agactaccta tttgaagtga tgcaggccgt tgaacaggtt attctgaaga
      901 agctggatgg catcccagac tgtgacatta gcccagtgca gattcgcaaa tgcacagaga
      961 agtttctttg cttcatgaaa ggacattttg ataacctttt tagcaaaatg gagcaactgt
     1021 ttttgcagct gattttacgt attccctcaa acatcttgct tcctgaagat aaatgtaagg
     1081 agacacctta tagtgaggaa gattttcagc atctccagaa agaaattgaa cagttacagg
     1141 agaagtacaa gactgaatta tgtactaagc aggcccttct tgcagaatta gaagagcaaa
     1201 aaattgttca ggccaaactc aaacagacgt tgactttctt tgatgagctt cataatgttg
     1261 gcagagatca tgggactagt gattttaggg agagtttagt atccctggtt cagaactcca
     1321 gaaaactaca gaacattaga gacaatgtgg aaaaggaatc gaaacgactg aaaatatcta
     1381 tgctagcagg gagcactgaa ttctctagag gagtgcaggt ggaaaccatc tccccaggag
     1441 acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc gggatgcttg
     1501 aagatggaaa gaaatttgat tcctcccggg acagaaacaa gccctttaag tttatgctag
     1561 gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcaga
     1621 gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca
     1681 tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa actagaggcg
     1741 tgcaggtcga gaccatcagc cccggcgacg gccgcacctt tcccaagaga ggccagactt
     1801 gcgtggtcca ctacaccggc atgctggagg acggcaagaa gttcgacagc agccgcgacc
     1861 gcaacaagcc cttcaagttc atgctgggca aacaggaagt gatccgcggc tgggaggaag
     1921 gcgtggctca gatgagcgtg gggcagcggg ccaagctgac catcagcccc gactatgcct
     1981 acggcgccac cggccacccc ggcatcatcc ccccccacgc caccctggtg ttcgacgtgg
     2041 agctgctgaa gctggagact agtgaattcg gagtgcaggt ggaaaccatc tccccaggag
     2101 acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc gggatgcttg
     2161 aagatggaaa gaaatttgat tcctcccggg acagaaacaa gccctttaag tttatgctag
     2221 gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcaga
     2281 gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca
     2341 tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa tgatctagag
     2401 ggccctattc atcgataagc ttgatatcga attcctgcag cccgggggat ccgcccctct
     2461 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     2521 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     2581 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     2641 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     2701 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     2761 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     2821 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     2881 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc
     2941 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     3001 gttttccttt gaaaaacacg atgataatat ggccacaacc atgaccgagt acaagcccac
     3061 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt
     3121 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac
     3181 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     3241 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     3301 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     3361 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     3421 cgtcggcgtg tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     3481 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     3541 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     3601 accgcgcacc tggtgcatga cccgcaagcc cggtgcctag ccggagaccg aaaggagcgc
     3661 acgaccccat ggctccgacc gaagccgacc cgggcggccc cgccgacccc gcacccgccc
     3721 ccgaggccca ccgactctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
     3781 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     3841 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     3901 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcactagt
     3961 ttcgcgttaa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     4021 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     4081 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     4141 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtta
     4201 tctagatccg gtggatctat ttacccgggg agcatgtcaa ggtcaaaatc gtcaagggca
     4261 tcggctggga gcatgtctaa gtcaaaatcg tcaagggcgt cggtcggccc gccgctttcg
     4321 cactttagct gtttctccag gccacatatg attagttcca ggccgaaaag gaaggcaggt
     4381 tcggctccct gccggtcgaa cagctcaatt gcttgtctca gaagtggggg catagaatcg
     4441 gtggtaggtg tctctctttc ctcttttgct acttgatgct cctgttcctc caatacgcag
     4501 cccagtgtaa agtggcccac ggcggacaga gcgtacagtg cgttctccag ggagaagcct
     4561 tgctgacaca ggaacgcgag ctgattttcc agggtttcgt actgtttctc tgttgggcgg
     4621 gtgccgagat gcactttagc cccgtcgcga tgtgagagga gagcacagcg gtatgacttg
     4681 gcgttgttcc gcagaaagtc ttgccatgac tcgccttcca gggggcagaa gtgggtatga
     4741 tgcctgtcca gcatctcgat tggcagggca tcgagcaggg cccgcttgtt cttcacgtgc
     4801 cagtacaggg taggctgctc aactcccagc ttttgagcga gtttccttgt cgtcaggcct
     4861 tcgataccga caccattgag taattccaga gctccgttta tgactttgct cttgtccagc
     4921 ctagacatgg tggctagcgt ctaacaaaaa agccaaaaac ggccagaatt tagcggacaa
     4981 tttactagtc taacactgaa aattacatat tgacccaaat gattacattt caaaaggtgc
     5041 ctaaaaaact tcacaaaaca cactcgccaa ccccgagcgc atagttcaaa accggagctt
     5101 cagctactta agaagatagg tacataaaac cgaccaaaga aactgacgcc tcacttatcc
     5161 ctcccctcac cagaggtccg gcgcctgtcg attcaggaga gcctacccta ggcccgaacc
     5221 ctgcgtcctg cgacggagaa aagcctaccg cacacctacc ggcaggtggc cccaccctgc
     5281 attataagcc aacagaacgg gtgacgtcac gacacgacga gggcgcgcgc tcccaaaggt
     5341 acgggtgcac tgcccaacgg caccgccata actgccgccc ccgcaacaga cgacaaaccg
     5401 agttctccag tcagtgacaa acttcacgtc agggtcccca gatggtgccc cagcccatct
     5461 cacccgaata agagctttcc cgcattagcg aaggcctcaa gaccttgggt tcttgccgcc
     5521 caccatgccc cccaccttgt ttcaacgacc tcacagcccg cctcacaagc gtcttccatt
     5581 caagactcgg gaacagccgc cattttgctg cgctcccccc aacccccagt tcagggcaac
     5641 cttgctcgcg gacccagact acagcccttg gcggtctctc cacacgcttc cgtcccaccg
     5701 agcggcccgg cggccacgaa agccccggcc agcccagcag cccgctactc accaagtgac
     5761 gatcacagcg atccacaaac aagaaccgcg acccaaatcc cggctgcgac ggaactagct
     5821 gtgccacacc cggcgcgtcc ttatataatc atcggcgttc accgccccac ggagatccct
     5881 ccgcagaatc gccgagaagg gactactttt cctcgcctgt tccgctctct ggaaagaaaa
     5941 ccagtgccct agagtcaccc aagtcccgtc ctaaaatgtc cttctgctga tactggggtt
     6001 ctaaggccga gtcttatgag cagcgggccg ctgtcctgag cgtccgggcg gaaggatcag
     6061 gacgctcgct gcgcccttcg tctgacgtgg cagcgctcgc cgtgaggagg ggggcgcccg
     6121 cgggaggcgc caaaacccgg cgcggaggcc acgcgtacta gacgagttta ctccctatca
     6181 gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
     6241 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     6301 tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
     6361 gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg
     6421 tttagtgaac cgtcagatcg caccgggtga ctctcttaag gtagccttgc agaagttggt
     6481 cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga
     6541 aactgggctt gtcgagacag agaagactct tgcgtttcgg tcgacttcta gagctctgga
     6601 ggcttgctga aggctgtatg ctagagacgt acagatgcgt ctcacaggac acaaggcctg
     6661 ttactagcac tcacatggaa caaatggccg aattcggcgc tagctgctga tatcgcatac
     6721 gcgtggacca gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
     6781 ggtgtcggta ccgcgggccc gggatccacc ggtcaccatg gcttctagaa tcctctggca
     6841 tgagatgtgg catgaaggcc tggaagaggc atctcgtttg tactttgggg aaaggaacgt
     6901 gaaaggcatg tttgaggtgc tggagccctt gcatgctatg atggaacggg gcccccagac
     6961 tctgaaggaa acatccttta atcaggccta tggtcgagat ttaatggagg cccaagagtg
     7021 gtgcaggaag tacatgaaat cagggaatgt caaggacctc ctccaagcct gggacctcta
     7081 ttatcatgtg ttccgacgaa tctcaaagac tagtccggtc gccaccatgg tgagcaaggg
     7141 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     7201 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     7261 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     7321 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     7381 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     7441 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     7501 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     7561 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     7621 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     7681 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     7741 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     7801 ccactccacc ggcggcatgg acgagctgta taaggccggc tccagctctg ccgaacagag
     7861 cttcctgttc tccagggagg aggcggacac gctcaggttg aaggtcgagg agctggaagg
     7921 cgagcggagt cggctggagg aggaaaagag gatgctggag gcacagctgg agcggcgagc
     7981 tctgcagggt gactatgacc agagcaggac caaagtgctg cacatgagcc tgaaccccac
     8041 cagtgtggcc aggcagcgcc tgcacgagga ccacagccag ctgcaggcgg agtgcgagcg
     8101 actgcgcggg ctcctgcgcg ccatggagag aggaggcacc gtcccagccg accttgaggc
     8161 tgccgccgcg agtctgccat cgtccaagga ggtggcagag ctgaagaagc aggtggagag
     8221 tgccgagctg aagaaccagc ggctcaagga ggttttccag accaagatcc aggagttccg
     8281 caaggcctgc tacacgctca ccggctacca gatcgacatc accacggaga accagtaccg
     8341 gctgacctcg ctgtacgccg agcacccagg cgactgcctc atcttcaagg ccaccagccc
     8401 ctcgggttcc aagatgcagc tactggagac agagttctca cacaccgtgg gcgagctcat
     8461 cgaggtgcac ctgcggcgcc aggacagcat ccctgccttc ctcagctcgc tcaccctcga
     8521 gctcttcagc cgccagaccg tggcgtaggg atccctatga tgtacaagta aacactcagg
     8581 tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc
     8641 actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     8701 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     8761 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     8821 tggtttagag tttggcaaca tatgccatat gctggctgcc atgaacaaag gtggctataa
     8881 agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc
     8941 ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc
     9001 ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca
     9061 gtcatagctg tccctcttct cttatgaaga tccctcgacc tgagcgtcga gctagcatat
     9121 ggatccatat aacttcgtat aatgtatgct atacgaagtt attacgatac cgtcaacttg
     9181 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9241 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaggta
     9301 cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     9361 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     9421 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     9481 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9541 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9961 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    10021 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10081 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    10141 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    10201 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    10261 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    10321 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10381 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    10441 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    10501 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    10561 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    10621 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10681 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10741 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10801 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10861 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10921 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10981 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    11041 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    11101 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    11161 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    11221 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    11281 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    11341 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    11401 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    11461 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc c
//
