LOCUS       sequence-8987-ex        7409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, 
  TITLE     Single-copy insertion of transgenes in Caenorhabditis elegans
  PUBMED    18953339
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCFJ151 - ttTi5605_MCS
FEATURES             Location/Qualifiers
     source          1..7409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(146..734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(226..245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(905..1696,1697..1765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1528..1547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1766..1870)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1938..1956
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1994..2016)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2116..2135
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             5908..5919
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgtgaaattg ttatccgctg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
       61 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag accaggaacc
      121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
      601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1021 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     1921 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     1981 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2041 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2101 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2161 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2221 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2281 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agcacgtgtg tttttgaaca
     2341 cggcgatatg tatctgtaga tcatccccaa ttttcaacaa tgaatctaaa tactgaactt
     2401 tttgtgtaat ctttagtgca gaaaaatgac caaaatcagc gattttccga ttatacccaa
     2461 aatatctcag ctcaatgagc acctataaaa atgtaaaaat aaagaaagta gtcgaaccat
     2521 attttgtaaa ggctaaaaaa ctgaataaat ttttatccat ccagcgcggc gttacaatcc
     2581 gtggcccatg tcacgcacgg ctttcaaaga aaaaaaaaca gtctgtttgc gcgacatcga
     2641 tctggcaaaa atcctaaaaa cgactatgcg tggctcactt tatattaata tgaaaaatac
     2701 aaacttggtg gtgcaggtgc tgcagaggcc atcgatgaag aagtggaggc cttgttgtcg
     2761 atctacaaaa ttgtatggtt gtttgagatt ttccttgaaa cgctctcaat cttggatatg
     2821 atataaattc tggataagat atgatggata tgatctatgt ttgatagagt gcatttgaaa
     2881 ggataacggc cctgcagaca tttttgaaaa aataacaaac atttaatttt ctcactcgtt
     2941 taggctattc ccctattttt gatattcctt cgcacatatg aaaactactt tttttcgaaa
     3001 ctgttaactc cagaatttat aaatctatag cccttacttg attatttatt atcatggtta
     3061 ctcacctcat gtctctttct cttttcaaga agtcttcgtt catatctgtt gtctctttcc
     3121 tccttttgaa tctctgcttt gctcttcgcc attgtttcct gaaaataatg taacttgaat
     3181 tgtgtaatat acttttttaa tttgaatttg gcttgtaacg cggaatcact acgtgcggga
     3241 tcatttctta ctagaaaacc cagaaaatgc catatttcac ttatctcggg gtcatttcta
     3301 attagaaaag ctacaaaaac catttctaat tacggtgact ttagaactgg tcgaaatagc
     3361 taattagaaa tgggtttttg ctaattagag gtgactctat gttatctgag taagcctcgt
     3421 ttgtatgtaa actcacagta ctaataaatg caagacaccc gggtttgtct agatatgaaa
     3481 taattgaaat atcaattctg acagacaata atggtaatct tgataaggag ttccacgccc
     3541 aggagaacac gttagttttc ttgtttttga ttgcgtgcgt tattttggag aaaaactcga
     3601 ttttttacaa aataattttt tgaaaggaac actgttcaat aagttttgtc ttttttctca
     3661 gttgtgatac ggttttttat tcttttttgt agttatacag aagaccgtta cgaaacagac
     3721 tgatatcgaa acaaacactg atcttacttg cacttataat acgactcact agtgggcaga
     3781 tcttcgaatg catcgcgcgc accgtacgtc tcgaggaatt cctgcaggat atctggatcc
     3841 acgaagcttc ccatggtgac gtcaccggtt ctagatacct aggtgagctc tggtaccctc
     3901 tagtcaaggc cttaagtgag tcgtattacg tagcccgggc ctagttctag acattctcta
     3961 atgaaaaaat ctttcagttg aaattgaaaa tgagttaaag ttggagtttt tattgaaaac
     4021 agatttccgt gtgattagtg tttttagcga gtgtgacagg acagcgaaaa aatatagaaa
     4081 caagggggga actgaaaagc ttaggaatgc attgaacatg agaaggggaa ggggaaggaa
     4141 caaactagac aggaattatt ggaatttaat cacatttgga gttttttttc tattcgacag
     4201 aataattatc cagaacattt ttgtattaaa tatttatgca tcatatgagt agtcggcttt
     4261 gttgtgcatg acgagtttgt tatcgacgaa atagaagctg tcagaacgag tctcgtttgg
     4321 attgttgatc atgtcgtcca ctgaaaaaga gattagtctt ttgaattgta ctttttagaa
     4381 taatgactca ctgagttgtt gagagagttg agggaactca tagatatgtt cacagttgtt
     4441 tcgtgaattc ggaatacaga atccgaattc aaagtcaaaa cacttcagaa ggcgatcttt
     4501 gaagaagtga cgttcgatca ttcggaaatg atggattggg atgtctccta ctttgaattc
     4561 cacagttgct ccgaccgttt tgagtttcaa aaagtttgga gcaaatctat aacgcacgta
     4621 tcttgccgac tcctgtggcg actcatcatt ctcctgatcg ttctccggtt tggcgatctc
     4681 gaaaagcact tgctcagtgt ccagatcacg gatttggaac ttggtgaact cgatgttata
     4741 gatgttcgca gatggggagc ataagaatcc taaatttatg ttttaaactg aaatccaaag
     4801 ggagcaagat accttgagtg attcccggaa gtgctaaaac gtcgttcgga gtgatttgag
     4861 ctttcttcgc aagctccgat tccgttgtga ttccttgttc ggtgcttggt ggtggccgtg
     4921 gcatctggaa atatggaaaa gttcaacaaa aagaaaagag aaaagaatga aatcggatat
     4981 caagagttag ttgagcggtt tctctagttt tctgagtctc acctgcgacg ggaaggtcgc
     5041 cgagccgggt ggaatcgatc gttgttgctc ggctttcata tcggtttggt tggaagcggc
     5101 tgaaaacgga aagaagtgga agaaggaaaa gagtgtggtg tgacaggaaa atggtaatta
     5161 gagggtgcca aataaccagc tatattttgt ttttttttga aaacattttt aaaaagaaaa
     5221 atacgataat gatatcagat ggatttccgg aaaactggta tgaaaaattt caaccttttt
     5281 gagtacatgt aatcaaaata cactttgtaa attatcattt ttattgaaac tccaccattt
     5341 ttctatttat aacgctaata atttgaaaaa gaaacctgtt gcgaaccgcg gggtgaatcc
     5401 caaaaacgaa tgcgttttgg tggagtgatt gattcgaatc gaagaagaaa aagaagaaga
     5461 cgtggaatag agagctcact cttaaccgag cagcacacac cgacagaaaa aaaaatgaaa
     5521 tgaatgaggg tcttcttctt cttcttcttc gaatgattga cagaaatggg aaaaagagga
     5581 agattgagaa gggaaaaagg aaggagaaaa gaagcagaag aagacgtcag agaggagagg
     5641 aacgagcgga aaagcagcgg gcgcaagtca tagaagtagc agagctgggg agaagaagac
     5701 actatccaag aaaggaatga cgagagagta tgcaaagggg tatagggtgc agacagaata
     5761 ggaacagaat aacagatgat gagccaagaa gagttgaaaa gggcgatgaa tttgtcatgt
     5821 aacttaattt gggtcaattt gagcatgatg aattgaaatc atcccttgtt gggagttaat
     5881 aaccggtttg ttatcagaaa ccctgtaata gaagggcgcc ctaactttga gccaattcat
     5941 cccggtttct gtcaaatata tcaaaaagtg gtcaactgac aaattgtttt tgatattata
     6001 ataaacattt tatccgttaa caattttcga atacttttta caaggacttg gataaattgg
     6061 ctcaaagacg aagtgagttt gctaccatca tggaatcagg aggacatttc gacaatgtca
     6121 tataatgttt gaaaaatgat tttaacaagg gtctcattcg agataagcaa tgctctgagc
     6181 aagtggctcg tcgtcttcaa gaatctcttg ttgatattca tttggatcct gttttgcacg
     6241 aagcatgtgc catggatatt cagagttttt gcaaaaaagt tccgccaggt caaacaatct
     6301 ttttaatttt tatatgatac ttcgggtgta tttttcttct ttttcctgtt cattttcgct
     6361 tttttctcac ttttttgtaa caaaatctct cacttttctg taagtcttaa caaaagttca
     6421 attggctcga tcaagtaaaa tttcggccgt atgaacgttt tcaaaggttt tcattttttt
     6481 ttaagttcat tcacaatatc atgaatctct cgtcctttgg tttccggtaa gtagcgaaat
     6541 aatataagca tacttataaa tgaaggaatt gaatatagaa taaggaaagc ataactacca
     6601 ataacggaat atagaggtaa tactgtaaat gtactgataa ccaccattac agtattcatt
     6661 gaatatgcca cagattgagc aatactacga tgtttttgag gtaccagttc agaggatata
     6721 aaccacgaaa ttggaccaac tccagttctg aaatttcatt attcgattaa atcaaaatat
     6781 atttgaaaaa cctcacccat aagtataacc gtagatgatc aatgcaacta ggcatccgta
     6841 tttcatcggg tcaatcagaa tacgtatttt tgcgaagaat acgaatgcaa ttagagctaa
     6901 attattgaga aatgtgaaga gaaggagaag cttgcgtcga ccaactctgt aaacttattc
     6961 atacaatttt gtcttcaaat tttgaacgtt taaaactatc ttattcaatg agttagcggg
     7021 tttttatagt gagttagttc acgattatct aataaaacta tgtccaaaca tcctaactgc
     7081 gaccgtattc tcactaactc gtcttaaaaa cgttgagatt attttaaacc cttcttcagt
     7141 acaactacag tactgatgca caaatacaat aataataata cattcagaca tactctgaac
     7201 aatgtaactt gtttttgtta tactgtggta acatttattg aaatgaaata taaccttact
     7261 tttcaatcac aattccacca gtaattgttc cagaaacata agctaaaccc attgcagttg
     7321 tactccattc agcaatttct gactcaacat ttgcattttc tagaaagaaa gtagaggata
     7381 aaagaagagc ccataatccg acagggccc
//
