LOCUS       sequence-89870-e        6102 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for inserting ubi-CadTS to Drosophila 
            melanogaster genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Cai D, Chen SC, Prasad M, He L, Wang X, Choesmel-Cadamuro V, Sawyer 
            JK, Danuser G, Montell DJ
  TITLE     Mechanical Feedback through E-Cadherin Promotes Direction Sensing 
            during Collective Cell Migration.
  JOURNAL   Cell. 2014 May 22;157(5):1146-59. doi: 10.1016/j.cell.2014.03.045.
  PUBMED    24855950
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.03"; journalName: "Cell"; date: "2014-05-22- 
            22"; volume: "157"; issue: "5"; pages: "1146-59"
COMMENT     Sequence Label: pUWR-CadTS
FEATURES             Location/Qualifiers
     source          1..6102
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4129..4836
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(4957..4959,4960..4962,4963..5673)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5002..5023)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     5610..5631
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgtccacca gtgtccagcg aatgtccaga agctaccact gcatcaatat gtcggcgacc
       61 ccgcaggccg gccacctaaa tcccgcccag caacagacgc accagcagca caagcgaaaa
      121 tgccgcgacc ttggcaggcg actaatcccc gcgagactcc tcctgggcgt tatcgtggcc
      181 atcagccttc tgtcacccgc cctcgcactt cactcgccgc cggacaaaaa cttttccggc
      241 gacaatcgta agcccgcctt caaaaactgc gccggatacg ctcctaaggt caaagaggag
      301 cagcccgaaa acacctacgt tctcaccgta gaggccgtcg accctgatcc cgaccaggtc
      361 attcgataca gcatcgtcca gtcgccgttc gagcgaccca agttctttat caatcccagc
      421 acgggcgtaa tcttcaccac ccacacattc gatcgtgacg agccgatcca cgagaagttc
      481 gtcttcgtca ccgtccaggc cacggataat ggtctgcccc cactggacga cgtttgcacc
      541 ttcaacgtta ccatcgagga catcaacgac aatgcacccg ccttcaacaa ggcgcgctac
      601 gacgaatcca tgtcggaaaa tgcccagccc gacgcggtgg tgatgactat cagcgccagt
      661 gacttcgacg atggcaacaa cagtttggtc gaatacgaga ttctgcggga gcgcgacttt
      721 cagtacttca agattgacaa ggagtcgggt attatctacc tgaaacgacc gattgacaag
      781 agacccggcc aatcgtatgc gataatcgtg cgggcatata acgtagtgcc agatccgcca
      841 caggatgcac aaatcgaagt gcgcatccgg gtggtggagt cctcaattaa gccgccatca
      901 tttgtgaatc cgatcgatac gcccatttac ctgaaggaga atctcaagaa ctttacacat
      961 ccgatcgcca cgttacgagc cgtttccaat atgccggaca agccggaggt aatcttcgaa
     1021 ctgaacaccg gccgcacgga gcagacaaac agcaagaaca cattcgtgtt caatcagatc
     1081 ggcaacgagg tgaccattag cctgggcaag actctggact acgaggccat cacagactac
     1141 accctcacca tgattgtgcg gaatacacac gagctgggca cagagcatca gatcaagatt
     1201 caggtggagg atgtcaacga taacatcccc tactacacgg aggtcaaatc gggcactatc
     1261 ctggagaacg aaccacccgg cacgcctgtt atgcaggtgc gtgccttcga catggacggc
     1321 acctcagcca ataatattgt gtcattcgag ctggccgaca atcgggagta cttcaccatc
     1381 gaccccaata ccggtaacat aaccgccctg acgacattcg atcgcgagga acgcgatttc
     1441 tataacgtaa aggttattgc cagcgacaat tcgccatcga gtctctttga taatggcgag
     1501 ccgaaccgtg gtcaccaagt gttccgcatc tccattggag acaagaacga ccacaagccg
     1561 cacttccagc aggacaaata tctggcagaa agactgctcg aggatgccaa tacgaatacc
     1621 gaggtcattg aggtaaaggc cgaagacgag gataacgcat cccagatcct atacagcatt
     1681 gagagcggta acgtgggtga tgcctttaag attggtctta agaccggcaa aatcacggtc
     1741 aaccagaagt tggactacga aacgatcacg gagtacgagc tgaaggtgcg tgcgttcgat
     1801 ggaatctacg acgactacac aacggtggtt atcaaaattg aagacgtgaa cgacaacccg
     1861 ccggtgttta agcaggacta cagcgtcacc attctggagg agaccacata tgacgactgc
     1921 atcctcactg tcgaagccta cgatccggat atcaaggatc gcaacgcaga tcagcatatc
     1981 gtctattcga ttcaccagaa cgatggaaac cgatggacca ttgacaacag tggctgcttg
     2041 cgtttggtca agacgctgga ccgcgatccg cccaatggtc acaagaactg gcaggttttg
     2101 atcaaggcta atgatgagga tggagtagga accacggtca gcacagtgaa agaggtcacc
     2161 gtcacgttga aggacatcaa cgacaatgct ccgttcctga tcaacgaaat gcctgtctac
     2221 tggcaggaga accgcaatcc cggacatgtt gtgcaactgc aggccaacga ctacgacgac
     2281 actccggggg caggtaactt caccttcggc atcgacagcg aagccacacc ggacatcaag
     2341 accaagttca gcatggatgg agactatttg cacgccaacg tccagtttga tcgggaggct
     2401 caaaaggagt actttatccc cattcgcatc agtgattctg gcgttccgcg acagagtgcc
     2461 gtaagcattc tgcacctggt gatcggcgat gtcaacgaca atgccatgag cgaaggatcg
     2521 tcgcgcatct tcatttacaa ttacaaggga gaagcgcctg aaaccgatat tggtcgcgtg
     2581 ttcgtagatg atctcgatga ctgggacttg gaagacaagt actttgaatg gaaagacctt
     2641 ccgcacgatc agtttaggct taatcccagc acgggtatga tcaccatgct ggttcacacc
     2701 gccgagggag agtacgacct gtcgttcgta gtcaccgagg actccatgtt cgtgccccgt
     2761 cactccgtag atgcttatgt caccgtggtg gtccgcgagc tccccgagga ggcggtggat
     2821 aagagcggca gcatccgatt catcaacgta accaaggagg agtttatcag tgtgccacgc
     2881 gacttccagt caccggatgc cctttcccta aaggatcgct tccagctctc gctggccaag
     2941 cttttcaata cgtcggtatc caacgtggac gtgttcactg tgctccagaa cgagaatcat
     3001 acgctcgatg tacgcttctc tgcccacggc tcaccctact atgctccgga gaaattaaat
     3061 ggaatagtgg cccagaatca gcagcgtttg gaaaacgaac tagacctgca gatgctgatg
     3121 gtgaacatcg acgagtgtct gattgagaag ttcaagtgcg aggaatcgtg caccaatgag
     3181 ctgcacaaga gctcggtgcc atacatgatc tactctaata caacatcgtt cgtgggcgta
     3241 aatgccttcg ttcaagcgca atgcgtatgc gaggctcctt taatgaggcg ttgtctaaac
     3301 ggaggatctc cgcggtacgg ggagaacgac gtgtgcgact gcatagacgg attcaccgga
     3361 ccccactgcg aactcgtctc cgtggcattc tatggctcgg ggtatgcctt ctacgagccc
     3421 attgccgcct gcaacaacac caagattagc ttggagatca cgccccagat tgatcagggc
     3481 ctgatcatgt acttgggacc acttaacttc aatccgttgc tggccatttc tgatttccta
     3541 gctctggagt tggacaatgg ctatccggtg ctgactgtgg actacggatc aggcgccatt
     3601 cgcatccgcc atcagcacat caagatggtg gccgaccgca cctaccaact ggacattatc
     3661 ctgcagcgta ccagcattga gatgactgtg gacaattgcc ggctatccac ctgccagaca
     3721 ctgggcgccc caattggccc caatgagttc ctcaatgtga acgcaccgct gcagttgggc
     3781 ggcactccag tagatctgga gcaacttgga cgccagctca attggacaca cgttcccaac
     3841 cagaagggtt tcttcggttg cattcgcaac ctgacaatta acgagcagac ttacaacttg
     3901 ggaatgccct ctgtgttccg aaacatcgac agcggttgcc agcagtcggt ggccgttgcg
     3961 ttcagttttg gaattgatag aaacttcatc attgcgatca tcgtatgcct cgcgctactg
     4021 ctgatcatcc tgttggcagt ggtggtgcag aagaagcaga agaatggctg gcacgaaaag
     4081 gacatcgacg acattcgcga gacgatcggt accagtgtgg tggaattcat ggtgagcaag
     4141 ggcgaggaga ccacaatggg cgtaatcaag cccgacatga agatcaagct gaagatggag
     4201 ggcaacgtga atggccacgc cttcgtgatc gagggcgagg gcgagggcaa gccctacgac
     4261 ggcaccaaca ccatcaacct ggaggtgaag gagggagccc ccctgccctt ctcctacgac
     4321 attctgacca ccgcgttcgc ctacggcaac agggccttca ccaagtaccc cgacgacatc
     4381 cccaactact tcaagcagtc cttccccgag ggctactctt gggagcgcac catgaccttc
     4441 gaggacaagg gcatcgtgaa ggtgaagtcc gacatctcca tggaggagga ctccttcatc
     4501 tacgagatac acctcaaggg cgagaacttc ccccccaacg gccccgtgat gcagaagaag
     4561 accaccggct gggacgcctc caccgagagg atgtacgtgc gcgacggcgt gctgaagggc
     4621 gacgtcaagc acaagctgct gctggagggc ggcggccacc accgcgttga cttcaagacc
     4681 atctacaggg ccaagaaggc ggtgaagctg cccgactatc actttgtgga ccaccgcatc
     4741 gagatcctga accacgacaa ggactacaac aaggtgaccg tttacgagag cgccgtggcc
     4801 cgcaactcca ccgacggcat ggacgagctg tacaaggggc caggtggtgc agggccaggt
     4861 ggtgcagggc caggtggtgc agggccaggt ggtgcagggc ccggtggtgc aggtccaggt
     4921 ggtgcaggtc caggtggtgc aggtccaggt ggtgctatgg tgagcaaggg cgaggagctg
     4981 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     5041 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc
     5101 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc
     5161 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     5221 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     5281 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     5341 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     5401 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     5461 cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc
     5521 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaaactg
     5581 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     5641 gggatcactc tcggcatgga cgagctgtac aagtatgcgg ccgctattaa ttacgaggac
     5701 gagggtggcg gcgagcggga tacggactat gatctgaatg tcctgcgcac gcagcccttc
     5761 tacgaggaga agctgtacaa agatcctcat gcgttacagg gtaacatgcg cgaccccaac
     5821 gacatacccg acatcgccga cttcctgggc gacaagaagg agaactgcga ccgggatgtg
     5881 ggcgccacaa ccgtggacga tgtgcggcac tacgcgtacg agggtgacgg caactccgat
     5941 ggcagcctct ccagtctggc gtcctgcacc gacgacggcg atctcaactt cgactacctg
     6001 tccaactttg gaccgcgctt ccgcaaattg gccgacatgt acggcgagga gccctcagac
     6061 acagactcca acgtggacga tgaccagggc tggcgcatct ag
//
