LOCUS       sequence-89874-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CUL3A-c013
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(98..121)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(122..139)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(140..160)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atcctcactt atcgtcgtca tccttgtaat
      121 catggtggtg atgatggtgc gattggaagt agaggttctc tgccagatac tcgggcgagc
      181 gactgttcag attcagaaag tattcgaagt cgcctgcaat ggtctgttta aacagacgat
      241 cgttattgaa ggactccagc agaaagcggt cgaaacggct cttcagatcg tccagaccct
      301 ggcggtagtc cacagggttt ttgccttcac cctcttcgga caccagcgcc ttgccctgct
      361 cacgcagata gctgctcata cattcgcaca tggttttcag accattcggc acacggctga
      421 acagcttgta catacagccc aggtcttcgg ttttaccgtt cttcagcata tgaaccaggc
      481 cgctgttttc catttcgaca atggttttca tgtgcttaga gatcagctcg cgttcgacca
      541 ctttcacaat tggctcttcg gttgacttat ccaggcaatg cataacgcgc tcaatctctt
      601 cgttgatacg agcttccact ttcttgatat aaactgatgc gctgttttcc gccaggaatt
      661 tctgcgattc catctgaaag aactctgcac tcatttccag aaagggcgcc tcgaagtcct
      721 cttcgtagac agaacggcct tccagaccca gaatcatcag catctgacaa gcgttgcgga
      781 tggcgccacg atcaacgacc tcgcccttgc gttcacgggc aatcatatcc agcagggtct
      841 ggcgcaggtg gtcacggatg cagccatagc gcacaacctg gtcacgaaaa atgatcagac
      901 ccaggttgta aacattttcg acgttattct gctgcacata aacgcgatcc atgtacatca
//
