LOCUS       sequence-89876-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: JMJD1CA-c257
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(96..119)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(120..137)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(138..158)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcggat cctcacttat cgtcgtcatc cttgtaatca
      121 tggtggtgat gatggtgcga ttggaagtag aggttctctg cattttcttc catatcctct
      181 acttcatcct cgtgtatctt caaggctctc accatttctt tgactgcatg atacaaaata
      241 tttttaacct gtagtttatc atcataattg atttcttcct tcaaaagtct cagttcctgt
      301 gttaaatgaa atgactctac aagatgttct ggagacacaa aatcttcagt tacctgaata
      361 cagctgtgaa aattctgaac ctgatgaagt gctcccgctg gcaaaacaat agcatcacca
      421 aggaactgaa taagagtaca ggttctgact ccatattctt caagcagcct ttgacggagc
      481 tttttgttca cataccaact ttggtcacgt attggatcat gttctggtag aacttcaagg
      541 ccttgttctt ttgaaatctt ttgaagaaat tcccttatct tgtcaacatc tttcccagca
      601 taaatatgcc acagagcacc aggtatttca cttgagtcat tcaatctttt ccttaaaatg
      661 tcatccaaat cttcttcctc aaatttcttg agaattcctg cttttgagag aatgccattt
      721 ccttttgcta tgccaacata aactagtata tttacaacat cagaaacttc aatatggaga
      781 tttgttgttc ctatatcatg atctttagca gcaactacac cataggcact gcacaacctg
      841 ggtcctagat caggacgtac aaaaaatcct ggcaaatgag aggccaaatt g
//
