LOCUS       sequence-8988-ex        7312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, 
  TITLE     Single-copy insertion of transgenes in Caenorhabditis elegans
  PUBMED    18953339
REFERENCE   2  (bases 1 to 7312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCFJ178 - cxTi10882_MCS
FEATURES             Location/Qualifiers
     source          1..7312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(43..631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(123..142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(802..1593,1594..1662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1425..1444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1663..1767)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1835..1853
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1891..1913)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2013..2032
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2226..2248
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2240..2257
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2241..2257
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2265..2285
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3333..3357
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5509..5520
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5669..5693)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7193..7209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7193..7209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7206..7228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gcaaaagacc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      481 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
      841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     1801 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     1861 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     1921 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     1981 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     2041 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     2101 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2161 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2221 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttatcaacta tgtataataa
     2281 agttgcggcc cattaggtca gacaaaaatt tctgatcttt aaaccttatt gattaaaact
     2341 aatttttgcc cgactttttt gacacatgca ctagacgctt gtactcaaaa tttggaaacc
     2401 ttgctcaaat ccactactca gatatgacag tagcatcgat aattgaacca ttatcctgaa
     2461 atcccctttc ctgtcccggt tcattttcca attttttcaa cggtggaaga aggggcaaat
     2521 tttataccaa aacgtctccg tttatgaccc accccctatg caaaataaac ggctctggga
     2581 gaaatagtac agaaaacgcg gagcctgctt gattccttct ccgaggcgac aaaaaacgca
     2641 tcattcgtat tgtaagatgc atgtgtggct ggcctctttt gaattttact ggttttttat
     2701 atttctacta atatttcgaa aaattttgta agtaaatcat tgattacgtt tacaaaaatt
     2761 tagaaacatt tcaaaatgat tacttctaaa ttaattatat ttacaggtta gttcgagtta
     2821 cttatgacct tccaatccgc catatccgcc aaggacgctc caattaattt tttagaatta
     2881 ttttttaaca catgtattat tttttaacaa taaattattc atgaaatcat caaatgtata
     2941 tgttctacta taatatttag gtcaaactat agaaaagaaa tccttgtttt tggaacaaag
     3001 gagttcagat cctgtgcatt tgatcttgga tccacatctt tggtccatgg gaaagcttgg
     3061 cactgatcaa gaaggattct tcaaatatct aaaattgccg gctgcccgta gaattccatc
     3121 agttttccag agttttagtg aaaatttgca tcccgttaga aacaatctac acaacggtaa
     3181 aaaggaccca ttatttttac cgtaccgttt tcctttatgg ccctctatgc aaaaaattat
     3241 agcgtggagg ggcagtacag aataataaag tgtgagaaaa aaaactgctc tgcgagaaat
     3301 agtacagcaa acgcggagca tgcttgattc tatccacttt gtacaagaaa gctgggttaa
     3361 tacgactcac tagtgggcag atcttcgaat gcatcgcgcg caccgtacgt ctcgaggaat
     3421 tcctgcagga tatctggatc cacgaagctt cccatggtga cgtcaccggt tctagatacc
     3481 taggtgagct ctggtaccct ctagtcaagg ccttaagtga gtcgtattac gtagcccggg
     3541 cctagttcta gacattctct aatgaaaaaa tctttcagtt gaaattgaaa atgagttaaa
     3601 gttggagttt ttattgaaaa cagatttccg tgtgattagt gtttttagcg agtgtgacag
     3661 gacagcgaaa aaatatagaa acaagggggg aactgaaaag cttaggaatg cattgaacat
     3721 gagaagggga aggggaagga acaaactaga caggaattat tggaatttaa tcacatttgg
     3781 agtttttttt ctattcgaca gaataattat ccagaacatt tttgtattaa atatttatgc
     3841 atcatatgag tagtcggctt tgttgtgcat gacgagtttg ttatcgacga aatagaagct
     3901 gtcagaacga gtctcgtttg gattgttgat catgtcgtcc actgaaaaag agattagtct
     3961 tttgaattgt actttttaga ataatgactc actgagttgt tgagagagtt gagggaactc
     4021 atagatatgt tcacagttgt ttcgtgaatt cggaatacag aatccgaatt caaagtcaaa
     4081 acacttcaga aggcgatctt tgaagaagtg acgttcgatc attcggaaat gatggattgg
     4141 gatgtctcct actttgaatt ccacagttgc tccgaccgtt ttgagtttca aaaagtttgg
     4201 agcaaatcta taacgcacgt atcttgccga ctcctgtggc gactcatcat tctcctgatc
     4261 gttctccggt ttggcgatct cgaaaagcac ttgctcagtg tccagatcac ggatttggaa
     4321 cttggtgaac tcgatgttat agatgttcgc agatggggag cataagaatc ctaaatttat
     4381 gttttaaact gaaatccaaa gggagcaaga taccttgagt gattcccgga agtgctaaaa
     4441 cgtcgttcgg agtgatttga gctttcttcg caagctccga ttccgttgtg attccttgtt
     4501 cggtgcttgg tggtggccgt ggcatctgga aatatggaaa agttcaacaa aaagaaaaga
     4561 gaaaagaatg aaatcggata tcaagagtta gttgagcggt ttctctagtt ttctgagtct
     4621 cacctgcgac gggaaggtcg ccgagccggg tggaatcgat cgttgttgct cggctttcat
     4681 atcggtttgg ttggaagcgg ctgaaaacgg aaagaagtgg aagaaggaaa agagtgtggt
     4741 gtgacaggaa aatggtaatt agagggtgcc aaataaccag ctatattttg tttttttttg
     4801 aaaacatttt taaaaagaaa aatacgataa tgatatcaga tggatttccg gaaaactggt
     4861 atgaaaaatt tcaacctttt tgagtacatg taatcaaaat acactttgta aattatcatt
     4921 tttattgaaa ctccaccatt tttctattta taacgctaat aatttgaaaa agaaacctat
     4981 tgcgaaccgc ggggtgaatc ccaaaaacga atgcgttttg gtggagtgat tgattcgaat
     5041 cgaagaagaa aaagaagaag acgtggaata gagagctcac tcttaaccga gcagcacaca
     5101 ccgacagaaa aaaaaatgaa atgaatgagg gtcttcttct tcttcttctt cgaatgattg
     5161 acagaaatgg gaaaaagagg aagattgaga agggaaaaag gaaggagaaa agaagcagaa
     5221 gaagacgtca gagaggagag gaacgagcgg aaaagcagcg ggcgcaagtc atagaagtag
     5281 cagagctggg gagaagaaga cactatccaa gaaaggaatg acgagagagt atgcaaaggg
     5341 gtatagggtg cagacagaat aggaacagaa taacagatga tgagccaaga agagttgaaa
     5401 agggcgatga atttgtcatg taacttaatt tgggtcaatt tgagcatgat gaattgaaat
     5461 catcccttgt tgggagttaa taaccggttt gttatcagaa accctgtaat agaagggcgc
     5521 cctaactttg agccaattca tcccggtttc tgtcaaatat atcaaaaagt ggtcaactga
     5581 caaattgttt ttgatattat aataaacatt ttatccgtta acaattttcg aatacttttt
     5641 acaaggactt ggataaattg gctcaaagag cctgcttttt tgtacaaact tgaattcaaa
     5701 aacgcagaac tcgtactgtt ggatgcctgt gtagcggtcc tctattgatt tttgctattt
     5761 tttgataatt ttactgtaaa ttatgagtta ttccaattaa cattcctagc taaatgtaag
     5821 ttagcgacca attttttagc aaccccattt tatgactttt cagaatatcg cctactgggt
     5881 caacgacact tgtttggaac taagggtctt cccaattttt tttaaatctt cataggcttg
     5941 acagaatcag ggttcttcaa gcagctttga gagggaatca attgcaacat ttgatgttaa
     6001 atttatattt tttatagttc tctggtgaaa tggggaccac cgcaaagccc cttttatgtt
     6061 tttaacttga ataaaatctt gtattaaatt gtgatttata gatctgtatg ggtaatttga
     6121 aggaaaagaa agggagctcc caaaagtgcc ttacagatcc aggcccaata aatgtaaact
     6181 attgggcagt agtatggcaa aaatcgtgcg gaatttgaaa aacctagaat taaaataaat
     6241 attcaacaca aatgttcgag atattttaag ttcggagtgt atatctagaa catattgtac
     6301 aagtaggtct acgggatcgt ctacgggaaa atttgatttc ctcgaagatg tatttaatga
     6361 attctctaat gttgcaggtc tttgtatttg acagaatgtt ggtttttgaa acttattcgc
     6421 cataattgct tggctactac atgtctcatg ccattgacac tatcctgtac tcgaaatttg
     6481 ttaagtaact acccaccgtc cacttatgac gagagggtca attaaggtca ctccacaaaa
     6541 gtaaatttga aaacacatga gaaaaagtga tctgaattcc cttgaagtgg ctaaaacaaa
     6601 gcagatgagg gatacgacgt atgcgactac tgagagataa ggtcgaaact tgataaggat
     6661 gctgcttacc tgcttagcaa gtttcttcgc acaggagaaa gtgacaagct aaactatcaa
     6721 attcatctaa aacctcaaaa aattattttg gatgaaataa tgttttattt tgagcaacgc
     6781 ataaggcaaa gaataaatta aaggcgatcc gcttagcggc gagttccgtc ctcgaagaag
     6841 attcctccat cgatggcgca gtactttgga cagattccac gctctcctgg ggttcctggt
     6901 ggtccgactg gtcctggctg tcctgggttt ccgtcggctc ctggcttgcc gtctggtcct
     6961 ggtggtccct ttggtccagg ttgtcctgaa agatgtggtg attagatatg gattgaaaaa
     7021 ctatgaaatg attacctggg gttccgtcgt ttcctggttg tccggatggt cctggtggtc
     7081 cgactggtcc tggctctccg gtggatcctg gctctccctt ttggactggc tcgcagacag
     7141 ctggaagtcc ctccaacttt ctatacaaag ttgatagctt ggcgtaatca tggtcatagc
     7201 tgtttcctgt gtgaaattgt tatccgctgg tatcagctca ctcaaaggcg gtaatacggt
     7261 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc ca
//
