LOCUS       sequence-89880-e        6099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for inserting ubi-CadTS control to Drosophila 
            melanogaster genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Cai D, Chen SC, Prasad M, He L, Wang X, Choesmel-Cadamuro V, Sawyer 
            JK, Danuser G, Montell DJ
  TITLE     Mechanical Feedback through E-Cadherin Promotes Direction Sensing 
            during Collective Cell Migration.
  JOURNAL   Cell. 2014 May 22;157(5):1146-59. doi: 10.1016/j.cell.2014.03.045.
  PUBMED    24855950
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.03"; journalName: "Cell"; date: "2014-05-22- 
            22"; volume: "157"; issue: "5"; pages: "1146-59"
COMMENT     Sequence Label: pUWR-CadTS-C
FEATURES             Location/Qualifiers
     source          1..6099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4552..5259
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(5380..5382,5383..5385,5386..6099)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5425..5446)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     6033..6054
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgtccacca gtgtccagcg aatgtccaga agctaccact gcatcaatat gtcggcgacc
       61 ccgcaggccg gccacctaaa tcccgcccag caacagacgc accagcagca caagcgaaaa
      121 tgccgcgacc ttggcaggcg actaatcccc gcgagactcc tcctgggcgt tatcgtggcc
      181 atcagccttc tgtcacccgc cctcgcactt cactcgccgc cggacaaaaa cttttccggc
      241 gacaatcgta agcccgcctt caaaaactgc gccggatacg ctcctaaggt caaagaggag
      301 cagcccgaaa acacctacgt tctcaccgta gaggccgtcg accctgatcc cgaccaggtc
      361 attcgataca gcatcgtcca gtcgccgttc gagcgaccca agttctttat caatcccagc
      421 acgggcgtaa tcttcaccac ccacacattc gatcgtgacg agccgatcca cgagaagttc
      481 gtcttcgtca ccgtccaggc cacggataat ggtctgcccc cactggacga cgtttgcacc
      541 ttcaacgtta ccatcgagga catcaacgac aatgcacccg ccttcaacaa ggcgcgctac
      601 gacgaatcca tgtcggaaaa tgcccagccc gacgcggtgg tgatgactat cagcgccagt
      661 gacttcgacg atggcaacaa cagtttggtc gaatacgaga ttctgcggga gcgcgacttt
      721 cagtacttca agattgacaa ggagtcgggt attatctacc tgaaacgacc gattgacaag
      781 agacccggcc aatcgtatgc gataatcgtg cgggcatata acgtagtgcc agatccgcca
      841 caggatgcac aaatcgaagt gcgcatccgg gtggtggagt cctcaattaa gccgccatca
      901 tttgtgaatc cgatcgatac gcccatttac ctgaaggaga atctcaagaa ctttacacat
      961 ccgatcgcca cgttacgagc cgtttccaat atgccggaca agccggaggt aatcttcgaa
     1021 ctgaacaccg gccgcacgga gcagacaaac agcaagaaca cattcgtgtt caatcagatc
     1081 ggcaacgagg tgaccattag cctgggcaag actctggact acgaggccat cacagactac
     1141 accctcacca tgattgtgcg gaatacacac gagctgggca cagagcatca gatcaagatt
     1201 caggtggagg atgtcaacga taacatcccc tactacacgg aggtcaaatc gggcactatc
     1261 ctggagaacg aaccacccgg cacgcctgtt atgcaggtgc gtgccttcga catggacggc
     1321 acctcagcca ataatattgt gtcattcgag ctggccgaca atcgggagta cttcaccatc
     1381 gaccccaata ccggtaacat aaccgccctg acgacattcg atcgcgagga acgcgatttc
     1441 tataacgtaa aggttattgc cagcgacaat tcgccatcga gtctctttga taatggcgag
     1501 ccgaaccgtg gtcaccaagt gttccgcatc tccattggag acaagaacga ccacaagccg
     1561 cacttccagc aggacaaata tctggcagaa agactgctcg aggatgccaa tacgaatacc
     1621 gaggtcattg aggtaaaggc cgaagacgag gataacgcat cccagatcct atacagcatt
     1681 gagagcggta acgtgggtga tgcctttaag attggtctta agaccggcaa aatcacggtc
     1741 aaccagaagt tggactacga aacgatcacg gagtacgagc tgaaggtgcg tgcgttcgat
     1801 ggaatctacg acgactacac aacggtggtt atcaaaattg aagacgtgaa cgacaacccg
     1861 ccggtgttta agcaggacta cagcgtcacc attctggagg agaccacata tgacgactgc
     1921 atactcactg tcgaagccta cgatccggat atcaaggatc gcaacgcaga tcagcatatc
     1981 gtctattcga ttcaccagaa cgatggaaac cgatggacca ttgacaacag tggctgcttg
     2041 cgtttggtca agacgctgga ccgcgatccg cccaatggtc acaagaactg gcaggttttg
     2101 atcaaggcta atgatgagga tggagtagga accacggtca gcacagtgaa agaggtcacc
     2161 gtcacgttga aggacatcaa cgacaatgct ccgttcctga tcaacgaaat gcctgtctac
     2221 tggcaggaga accgcaatcc cggacatgtt gtgcaactgc aggccaacga ctacgacgac
     2281 actccggggg caggtaactt caccttcggc atcgacagcg aagccacacc ggacatcaag
     2341 accaagttca gcatggatgg agactatttg cacgccaacg tccagtttga tcgcgaggct
     2401 caaaaggagt actttatccc cattcgcatc agtgattctg gcgttccgcg acagagtgcc
     2461 gtaagcattc tgcacctggt gatcggcgat gtcaacgaca atgccatgag cgagggatcg
     2521 tcgcgcatct tcatttacaa ttacaaggga gaagcgcctg aaaccgatat tggtcgcgtg
     2581 ttcgtagatg atctcgatga ctgggacttg gaggacaagt actttgaatg gaaagatctt
     2641 ccgcacgatc agtttaggct taatcccagc acgggtatga tcaccatgct ggttcacacc
     2701 gccgagggag agtacgacct gtcgttcgta gtcaccgagg actccatgtt cgtgccccgt
     2761 cactccgtag atgcttatgt caccgtggtg gtccgcgagc ttcccgagga ggcggtggat
     2821 aagagcggca gcatccgatt catcaacgta accaaggagg agtttatcag tgtgccacgc
     2881 gacttccagt caccggatgc gctatcccta aaggatcgct tgcagctctc gctggccaag
     2941 cttttcaata cgtcggtatc caacgtggac gtgttcactg tgctccaaaa cgagaatcat
     3001 acgctcgatg tacgcttctc tgcccacggc tcaccctact atgctccgga gaaattaaat
     3061 ggaatagtgg cccagaatca gcagcgtttg gaaaacgaac tagacctgca aatgctgatg
     3121 gtgaacatcg acgagtgtct gattgagaag ttcaagtgcg aggaatcgtg caccaatgag
     3181 ctgcacaaga gctcggtgcc atacatgatc tactctaata caacatcgtt cgtgggcgta
     3241 aatgccttcg ttcaagcgca atgcgtatgc gaggctcctt taatgaggcg ttgtctaaac
     3301 ggaggatctc cgcggtacgg ggagaacgac gtgtgcgact gcatagacgg attcaccgga
     3361 ccccactgcg aactcgtctc cgtggcattc tatggctcgg ggtatgcctt ctacgagccc
     3421 attgccgcct gcaacaacac caagattagc ttggagatca cgccccagat tgatcagggc
     3481 ctgatcatgt acttgggacc acttaacttc aatccgttgc tggccatttc tgatttccta
     3541 gctctggagt tggacaatgg ctatccggtg ctgactgtgg actacggatc aggcgccatt
     3601 cgcatccgcc atcagcacat caagatggtg gccgaccgca cctaccaact ggacattatc
     3661 ctgcagcgta ccagcattga gatgactgtg gacaattgcc ggctatccac ctgccagaca
     3721 ctgggcgccc caattggccc caatgagttc ctcaatgtga acgcaccgct gcagttgggc
     3781 ggcactccag tagatctgga gcaacttgga cgccagctca attggacaca cgttcccaac
     3841 cagaagggtt tcttcggttg cattcgcaac ctgacaatta acgagcagac ttacaacttg
     3901 ggaatgccct ctgtgttccg aaacatcgac agcggttgcc agcagtcggt ggccgttgcg
     3961 ttcagttttg gaattgatag aaacttcatc attgcgatca tcgtatgcct cgcgctactg
     4021 ctgatcatcc tgttggcagt ggtggtgcag aagaagcaga agaatggctg gcacgaaaag
     4081 gacatcgacg acattcgcga gacgatcatt aattacgagg acgagggtgg cggcgagcgg
     4141 gatacggact atgatctgaa tgtcctgcgc acgcagccct tctacgagga gaagctgtac
     4201 aaagatcctc atgcgttaca gggtaacatg cgcgacccca acgacatacc cgacatcgcc
     4261 gacttcctgg gcgacaagaa ggagaactgc gaccgggatg tgggcgccac aaccgtggac
     4321 gatgtgcggc actacgcgta cgagggtgac ggcaactccg atggcagcct ctccagtctg
     4381 gcgtcctgca ccgacgacgg cgatctcaac ttcgactacc tgtccaactt tggaccgcgc
     4441 ttccgcaaat tggccgacat gtacggcgag gagccctcag acacagactc caacgtggac
     4501 gatgaccagg gctggcgcat cggtaccggt ggaggtagtg tggtggaatt catggtgagc
     4561 aagggcgagg agaccacaat gggcgtaatc aagcccgaca tgaagatcaa gctgaagatg
     4621 gagggcaacg tgaatggcca cgccttcgtg atcgagggcg agggcgaggg caagccctac
     4681 gacggcacca acaccatcaa cctggaggtg aaggagggag cccccctgcc cttctcctac
     4741 gacattctga ccaccgcgtt cgcctacggc aacagggcct tcaccaagta ccccgacgac
     4801 atccccaact acttcaagca gtccttcccc gagggctact cttgggagcg caccatgacc
     4861 ttcgaggaca agggcatcgt gaaggtgaag tccgacatct ccatggagga ggactccttc
     4921 atctacgaga tacacctcaa gggcgagaac ttccccccca acggccccgt gatgcagaag
     4981 aagaccaccg gctgggacgc ctccaccgag aggatgtacg tgcgcgacgg cgtgctgaag
     5041 ggcgacgtca agcacaagct gctgctggag ggcggcggcc accaccgcgt tgacttcaag
     5101 accatctaca gggccaagaa ggcggtgaag ctgcccgact atcactttgt ggaccaccgc
     5161 atcgagatcc tgaaccacga caaggactac aacaaggtga ccgtttacga gagcgccgtg
     5221 gcccgcaact ccaccgacgg catggacgag ctgtacaagg ggccaggtgg tgcagggcca
     5281 ggtggtgcag ggccaggtgg tgcagggcca ggtggtgcag ggcccggtgg tgcaggtcca
     5341 ggtggtgcag gtccaggtgg tgcaggtcca ggtggtgcta tggtgagcaa gggcgaggag
     5401 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     5461 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg
     5521 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac
     5581 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     5641 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     5701 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     5761 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     5821 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     5881 atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc
     5941 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaaa
     6001 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     6061 gccgggatca ctctcggcat ggacgagctg tacaagtag
//
