LOCUS       sequence-89882-e        5277 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for inserting ubi-Cad-mTFP to Drosophila 
            melanogaster genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Cai D, Chen SC, Prasad M, He L, Wang X, Choesmel-Cadamuro V, Sawyer 
            JK, Danuser G, Montell DJ
  TITLE     Mechanical Feedback through E-Cadherin Promotes Direction Sensing 
            during Collective Cell Migration.
  JOURNAL   Cell. 2014 May 22;157(5):1146-59. doi: 10.1016/j.cell.2014.03.045.
  PUBMED    24855950
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.03"; journalName: "Cell"; date: "2014-05-22- 
            22"; volume: "157"; issue: "5"; pages: "1146-59"
COMMENT     Sequence Label: pUWR-Cad-mTFP
FEATURES             Location/Qualifiers
     source          1..5277
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4138..4845
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 atgtccacca gtgtccagcg aatgtccaga agctaccact gcatcaatat gtcggcgacc
       61 ccgcaggccg gccacctaaa tcccgcccag caacagacgc accagcagca caagcgaaaa
      121 tgccgcgacc ttggcaggcg actaatcccc gcgagactcc tcctgggcgt tatcgtggcc
      181 atcagccttc tgtcacccgc cctcgcactt cactcgccgc cggacaaaaa cttttccggc
      241 gacaatcgta agcccgcctt caaaaactgc gccggatacg ctcctaaggt caaagaggag
      301 cagcccgaaa acacctacgt tctcaccgta gaggccgtcg accctgatcc cgaccaggtc
      361 attcgataca gcatcgtcca gtcgccgttc gagcgaccca agttctttat caatcccagc
      421 acgggcgtaa tcttcaccac ccacacattc gatcgtgacg agccgatcca cgagaagttc
      481 gtcttcgtca ccgtccaggc cacggataat ggtctgcccc cactggacga cgtttgcacc
      541 ttcaacgtta ccatcgagga catcaacgac aatgcacccg ccttcaacaa ggcgcgctac
      601 gacgaatcca tgtcggaaaa tgcccagccc gacgcggtgg tgatgactat cagcgccagt
      661 gacttcgacg atggcaacaa cagtttggtc gaatacgaga ttctgcggga gcgcgacttt
      721 cagtacttca agattgacaa ggagtcgggt attatctacc tgaaacgacc gattgacaag
      781 agacccggcc aatcgtatgc gataatcgtg cgggcatata acgtagtgcc agatccgcca
      841 caggatgcac aaatcgaagt gcgcatccgg gtggtggagt cctcaattaa gccgccatca
      901 tttgtgaatc cgatcgatac gcccatttac ctgaaggaga atctcaagaa ctttacacat
      961 ccgatcgcca cgttacgagc cgtttccaat atgccggaca agccggaggt aatcttcgaa
     1021 ctgaacaccg gccgcacgga gcagacaaac agcaagaaca cattcgtgtt caatcagatc
     1081 ggcaacgagg tgaccattag cctgggcaag actctggact acgaggccat cacagactac
     1141 accctcacca tgattgtgcg gaatacacac gagctgggca cagagcatca gatcaagatt
     1201 caggtggagg atgtcaacga taacatcccc tactacacgg aggtcaaatc gggcactatc
     1261 ctggagaacg aaccacccgg cacgcctgtt atgcaggtgc gtgccttcga catggacggc
     1321 acctcagcca ataatattgt gtcattcgag ctggccgaca atcgggagta cttcaccatc
     1381 gaccccaata ccggtaacat aaccgccctg acgacattcg atcgcgagga acgcgatttc
     1441 tataacgtaa aggttattgc cagcgacaat tcgccatcga gtctctttga taatggcgag
     1501 ccgaaccgtg gtcaccaagt gttccgcatc tccattggag acaagaacga ccacaagccg
     1561 cacttccagc aggacaaata tctggcagaa agactgctcg aggatgccaa tacgaatacc
     1621 gaggtcattg aggtaaaggc cgaagacgag gataacgcat cccagatcct atacagcatt
     1681 gagagcggta acgtgggtga tgcctttaag attggtctta agaccggcaa aatcacggtc
     1741 aaccagaagt tggactacga aacgatcacg gagtacgagc tgaaggtgcg tgcgttcgat
     1801 ggaatctacg acgactacac aacggtggtt atcaaaattg aagacgtgaa cgacaacccg
     1861 ccggtgttta agcaggacta cagcgtcacc attctggagg agaccacata tgacgactgc
     1921 atcctcactg tcgaagccta cgatccggat atcaaggatc gcaacgcaga tcagcatatc
     1981 gtctattcga ttcaccagaa cgatggaaac cgatggacca ttgacaacag tggctgcttg
     2041 cgtttggtca agacgctgga ccgcgatccg cccaatggtc acaagaactg gcaggttttg
     2101 atcaaggcta atgatgagga tggagtagga accacggtca gcacagtgaa agaggtcacc
     2161 gtcacgttga aggacatcaa cgacaatgct ccgttcctga tcaacgaaat gcctgtctac
     2221 tggcaggaga accgcaatcc cggacatgtt gtgcaactgc aggccaacga ctacgacgac
     2281 actccggggg caggtaactt caccttcggc atcgacagcg aagccacacc ggacatcaag
     2341 accaagttca gcatggatgg agactatttg cacgccaacg tccagtttga tcgggaggct
     2401 caaaaggagt actttatccc cattcgcatc agtgattctg gcgttccgcg acagagtgcc
     2461 gtaagcattc tgcacctggt gatcggcgat gtcaacgaca atgccatgag cgaaggatcg
     2521 tcgcgcatct tcatttacaa ttacaaggga gaagcgcctg aaaccgatat tggtcgcgtg
     2581 ttcgtagatg atctcgatga ctgggacttg gaagacaagt actttgaatg gaaagacctt
     2641 ccgcacgatc agtttaggct taatcccagc acgggtatga tcaccatgct ggttcacacc
     2701 gccgagggag agtacgacct gtcgttcgta gtcaccgagg actccatgtt cgtgccccgt
     2761 cactccgtag atgcttatgt caccgtggtg gtccgcgagc tccccgagga ggcggtggat
     2821 aagagcggca gcatccgatt catcaacgta accaaggagg agtttatcag tgtgccacgc
     2881 gacttccagt caccggatgc cctttcccta aaggatcgct tccagctctc gctggccaag
     2941 cttttcaata cgtcggtatc caacgtggac gtgttcactg tgctccagaa cgagaatcat
     3001 acgctcgatg tacgcttctc tgcccacggc tcaccctact atgctccgga gaaattaaat
     3061 ggaatagtgg cccagaatca gcagcgtttg gaaaacgaac tagacctgca gatgctgatg
     3121 gtgaacatcg acgagtgtct gattgagaag ttcaagtgcg aggaatcgtg caccaatgag
     3181 ctgcacaaga gctcggtgcc atacatgatc tactctaata caacatcgtt cgtgggcgta
     3241 aatgccttcg ttcaagcgca atgcgtatgc gaggctcctt taatgaggcg ttgtctaaac
     3301 ggaggatctc cgcggtacgg ggagaacgac gtgtgcgact gcatagacgg attcaccgga
     3361 ccccactgcg aactcgtctc cgtggcattc tatggctcgg ggtatgcctt ctacgagccc
     3421 attgccgcct gcaacaacac caagattagc ttggagatca cgccccagat tgatcagggc
     3481 ctgatcatgt acttgggacc acttaacttc aatccgttgc tggccatttc tgatttccta
     3541 gctctggagt tggacaatgg ctatccggtg ctgactgtgg actacggatc aggcgccatt
     3601 cgcatccgcc atcagcacat caagatggtg gccgaccgca cctaccaact ggacattatc
     3661 ctgcagcgta ccagcattga gatgactgtg gacaattgcc ggctatccac ctgccagaca
     3721 ctgggcgccc caattggccc caatgagttc ctcaatgtga acgcaccgct gcagttgggc
     3781 ggcactccag tagatctgga gcaacttgga cgccagctca attggacaca cgttcccaac
     3841 cagaagggtt tcttcggttg cattcgcaac ctgacaatta acgagcagac ttacaacttg
     3901 ggaatgccct ctgtgttccg aaacatcgac agcggttgcc agcagtcggt ggccgttgcg
     3961 ttcagttttg gaattgatag aaacttcatc attgcgatca tcgtatgcct cgcgctactg
     4021 ctgatcatcc tgttggcagt ggtggtgcag aagaagcaga agaatggctg gcacgaaaag
     4081 gacatcgacg acattcgcga gacgatcggt accggtggag gtagtgtggt ggaattcatg
     4141 gtgagcaagg gcgaggagac cacaatgggc gtaatcaagc ccgacatgaa gatcaagctg
     4201 aagatggagg gcaacgtgaa tggccacgcc ttcgtgatcg agggcgaggg cgagggcaag
     4261 ccctacgacg gcaccaacac catcaacctg gaggtgaagg agggagcccc cctgcccttc
     4321 tcctacgaca ttctgaccac cgcgttcgcc tacggcaaca gggccttcac caagtacccc
     4381 gacgacatcc ccaactactt caagcagtcc ttccccgagg gctactcttg ggagcgcacc
     4441 atgaccttcg aggacaaggg catcgtgaag gtgaagtccg acatctccat ggaggaggac
     4501 tccttcatct acgagataca cctcaagggc gagaacttcc cccccaacgg ccccgtgatg
     4561 cagaagaaga ccaccggctg ggacgcctcc accgagagga tgtacgtgcg cgacggcgtg
     4621 ctgaagggcg acgtcaagca caagctgctg ctggagggcg gcggccacca ccgcgttgac
     4681 ttcaagacca tctacagggc caagaaggcg gtgaagctgc ccgactatca ctttgtggac
     4741 caccgcatcg agatcctgaa ccacgacaag gactacaaca aggtgaccgt ttacgagagc
     4801 gccgtggccc gcaactccac cgacggcatg gacgagctgt acaaggggca ggcggccgct
     4861 attaattacg aggacgaggg tggcggcgag cgggatacgg actatgatct gaatgtcctg
     4921 cgcacgcagc ccttctacga ggagaagctg tacaaagatc ctcatgcgtt acagggtaac
     4981 atgcgcgacc ccaacgacat acccgacatc gccgacttcc tgggcgacaa gaaggagaac
     5041 tgcgaccggg atgtgggcgc cacaaccgtg gacgatgtgc ggcactacgc gtacgagggt
     5101 gacggcaact ccgatggcag cctctccagt ctggcgtcct gcaccgacga cggcgatctc
     5161 aacttcgact acctgtccaa ctttggaccg cgcttccgca aattggccga catgtacggc
     5221 gaggagccct cagacacaga ctccaacgtg gacgatgacc agggctggcg catctag
//
