LOCUS       sequence-8989-ex        7077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, 
  TITLE     Single-copy insertion of transgenes in Caenorhabditis elegans
  PUBMED    18953339
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJL43.1 - Pglh-2::MosTase::glh-2utr
FEATURES             Location/Qualifiers
     source          1..7077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2218..2234
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2219..2235
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6385..6401)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6385..6401)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6433..6452)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6434..6452)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6462..6479)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6462..6478)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6471..6493)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6620..7075
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6707..6726)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6917..6938
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg
     2221 aggtcgacgg tatcgataag cttgatatcg aattcatatt accattattc ttgaactttt
     2281 atagtctttg gtagtggttt cgtgtagaaa ggcattggag tcgaaaggtt tgaaagtggt
     2341 tcagaagaat ccgagtcgaa atgaatccca gaatctacaa aaacttttat taaacaattt
     2401 agagcttttg agagtgtcat gttaaatttg cattcataac tgttttgtta tctgtttcta
     2461 ctaattagtt ccaccgaatc aggatgcagt gagcgaaacc gaaatcgtga atttcggttt
     2521 cgcccacagg ggacgaaaag taggttacta atatgtgaag cttttaaaaa ctccataaat
     2581 tttgtgaact ccacagctag gatcaagggt gccgatttat cgtttttcag gaaaacattt
     2641 ttttgtttac atttttttaa aaagttgcgg gttttctcgt tttagaataa attcatgttt
     2701 ccttaatttt tttttcatca acacaaaaaa ttgattatta aacattttct taaaaaattt
     2761 tccgattttt cgtttttcag gaaaatcgaa attttttcgc ttttccgata tttcgaaaaa
     2821 tatttcattt ttagagttta tcttcttttt agaagaaaat tatgttttct tatgattttt
     2881 cccatcaaca aaacaataat tttttgaaac tgatttttct ttgttaactt tctaaatttt
     2941 tttatttttc aggaaaatcg aacaatcgaa aattgttcat ttttcatgat accctaaaaa
     3001 tataaagttt gatcgatata atctatttca atcttatgaa aatttttaaa ataaactgac
     3061 cagaatcgga tgttgcttcg gtagctgaga ggttccattg ttcttcctca tcgacttcat
     3121 catcactatc ttcttcaatc gttgttacta cccattgttt tccaaccaat ttttcattgc
     3181 acttgaaatt ttcaagggct ttcatactac tttgatacat ttccaatgcc gaattatgct
     3241 cgttagtcat caaatcctca gcaggagaaa caccatccga gaaggaatac tttccaaaac
     3301 caacagagaa taaactttga ttttccatat caaatccaga agacttgtag tttgatattg
     3361 gtctagaaat ctggctgcag cgagtttcag agttacagcg agcaaaatca tattgtatca
     3421 gagccgtgaa gtacgtgaat ttcttgtatg aaaccagttt ttgaagctgt ttgagaatga
     3481 aaatatgttc atcgcaaggt ttttcagcat actttgaagc gtcacgtggg ttcagaatga
     3541 gttttccgtc cggtaagatc tcaatcaacg tttcagtgtt aagctctggt tgtgtgacat
     3601 actgcaattt tttaattgaa ggcaaaattt gatgttcaaa caaatttacg ttgaaaatcg
     3661 ccgtccgaat cgcgtatact tcgttaaaat gaacgttgaa tgaccgaagg gattgaagaa
     3721 tacaagctaa aacgtcgttg gcagtattcg tgatatgaac ccttaagaat attgtttaaa
     3781 tttggccaaa ttcagagtta ttaccattga atcagaagtt gaataatcca tgatttttct
     3841 tccataggca acggttctgc gaccattgca atcattgatt ggcatgaaat gtgtgccata
     3901 tcgtcgtcct tcattgtatg gcttctcagt gactcgataa tttttttcgc gaggggttcg
     3961 attccatcct tgctttgata atactgagtt atgtggctga gaagaactcc tcctcctcgc
     4021 ggacgaggca aatagtcttg cattgaaagt tcgttgagag catgaattgc gtccagtaca
     4081 ctaatattca tttctgaaaa aattaattaa attgtaaatt aaatatgcca aaagataaac
     4141 gaacgtttaa gtttaaaaag gtctccagat taattaggga aactattttc agaaagaaaa
     4201 attctgaagg ttccacaaag tattactggt atggcaaaaa gtgcgatgca aggatactga
     4261 aattgtattc agaaaatccc aaattgagtt tttaaaaggc tcatacgagc agcataggac
     4321 aagggtctcg ccacgttcgt cctctacagt actcccgcgc ggcaaatttc aaatcttttg
     4381 cgcgcgagtt tctgagtttc cattcattta aaattttttg gtttgcaaac gccaaacttg
     4441 tactttttcc tggaagttcg aagacgcgtt atgtcgagtt tcgtgccgaa taaagagcaa
     4501 acgcggacag tattaatttt ctgttttcat ttgaagaaaa cagctgcgga atcgcaccga
     4561 atgcttgttg aagcctttgg cgaacaagta ccaactgtga aaacgtgtga acggtggttt
     4621 caacgcttca aaagtggtga ttttgacgtc gacgacaaag agcacggaaa accgccaaaa
     4681 aggtacgaag acgccgaact gcaagcatta ttggatgaag acgatgctca aacgtaagtt
     4741 taaacatata tactaactaa cccatggatt atttaaattt tcaggcaaaa acaactcgca
     4801 gagcagttgg aagtaagtca acaagcagtt tccaatcgct tgcgagagat gggaaagatt
     4861 cagaaggtcg gtagatgggt gccacatgag ttgaacgaga ggcagatgga gaggcgcaaa
     4921 aacacatgcg aaattttgct ttcacgatac aaaaggaagt cgtttttgca tcgtatcgtt
     4981 actggagatg aaaaatggat cttttttgtt aatcctaaac gtaaaaagtc atacgttgat
     5041 cctggacaac cggccacatc gactgctcga ccgaatcgct ttggcaagaa gacgatgctc
     5101 tgtgtttggt gggatcagag cggtgtcatt tactatgagc tcttgaaacc cggcgaaacg
     5161 gtgaatacgg cacgctacca acaacaattg atcaatttga accgtgcgct tcagagaaaa
     5221 cgaccggaat atcaaaaaag acaacacagg gtcatttttc tccatgacaa cgctccatca
     5281 catacggcaa gagcggttcg cgacacgttg gaaacactca attgggaagt gcttccgcat
     5341 gcggcttact caccagacct ggccccatcc gattaccacc tattcgcttc gatgggacac
     5401 gcactcgctg agcagcgctt cgattcttac gaaagtgtga aaaaatggct cgatgaatgg
     5461 ttcgccgcaa aagacgatga gttctactgg cgtggaatcc acaaattgcc cgagagatgg
     5521 gaaaaatgtg tcgcgagcga cggcaaatac tttgaatagc tagcatgtat ctgcatatcc
     5581 ttcaaatgtt gtccatattg tatcagtaaa ttataatgcc ccctttttat atattctcct
     5641 tatcatgtaa atgttctatt gattttttgc tggtgtaaaa cgtttttata gtaaatcctc
     5701 tccacagtga atttgttaaa ttgtatcaaa acaagacatt gattaaattt gttcgagact
     5761 tgtggattgt aaaatatttt gcagaaaatg gcaatattca tttagaaaca ttacttccat
     5821 aataggaaaa actatttatg agttttcatg tgcctatgca agttcccact ctgtgtgaat
     5881 ttcatcatac aaattgagca aacataaggt ttatgtccag tatgggttct cagatgtttt
     5941 gtaagagttg agctgtcggt gaatgccttc tcacattgag cacatggata cggtctctct
     6001 ccagtgtgtg ttctgaaata atgaaaaata gtcttgcaat atgtttctac tattcgagaa
     6061 aaaatgtaat tttttttctt atggtaacct gcaatttata tattttttat ttgccgttat
     6121 tgacattatt ataagaaatt ggaggatatc acaaatatcg taaattctcc aaaattttga
     6181 cacttcactt taaagacaaa tgcggttttt ccgagtgtgt ctcgccacga tctcagtcat
     6241 aatggtcgct acaggtgcca aactttaggc atttgttata tttgatgcag aaaaccaagc
     6301 acatgaaaat taaaattgtc aagtagacaa tgaaatccag acattgttat gattcatttt
     6361 tttgcgaatt cctgcagccc gggggatcca ctagttctag agcggccgcc accgcggtgg
     6421 agctccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     6481 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     6541 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     6601 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6661 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6721 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6781 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6841 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6901 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6961 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7021 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttag
//
