LOCUS       sequence-89892-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 588, Emission: 618.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: HcRed1-C1
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..718
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     misc_feature    719..784
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcggcctgc tgaaggagag
       61 tatgcgcatc aagatgtaca tggagggcac cgtgaacggc cactacttca agtgcgaggg
      121 cgagggcgac ggcaacccct tcgccggcac ccagagcatg agaatccacg tgaccgaggg
      181 cgcccccctg cccttcgcct tcgacatcct ggccccctgc tgcgagtacg gcagcaggac
      241 cttcgtgcac cacaccgccg agatccccga cttcttcaag cagagcttcc ccgagggctt
      301 cacctgggag agaaccacca cctacgagga cggcggcatc ctgaccgccc accaggacac
      361 cagcctggag ggcaactgcc tgatctacaa ggtgaaggtg cacggcacca acttccccgc
      421 cgacggcccc gtgatgaaga acaagagcgg cggctgggag cccagcaccg aggtggtgta
      481 ccccgagaac ggcgtgctgt gcggccggaa cgtgatggcc ctgaaggtgg gcgaccggca
      541 cctgatctgc caccactaca ccagctaccg gagcaagaag gccgtgcgcg ccctgaccat
      601 gcccggcttc cacttcaccg acatccggct ccagatgctg cggaagaaga aggacgagta
      661 cttcgagctg tacgaggcca gcgtggcccg gtacagcgac ctgcccgaga aggccaactc
      721 cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg
      781 atccaccgga tctagataac tgatcataat cagccatacc acatttgtag aggttttact
      841 tgctttaa
//
