LOCUS       sequence-89894-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 507 / 583, Emission: 
            517 / 593.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Habuchi S, Tsutsui H, Kochaniak AB, Miyawaki A, van Oijen AM
  TITLE     mKikGR, a monomeric photoswitchable fluorescent protein.
  JOURNAL   PLoS One. 2008;3(12):e3944. doi: 10.1371/journal.pone.0003944. Epub 
            2008 Dec 15.
  PUBMED    19079591
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2008"; volume: "3(12):e3944. doi"; pages: " 
            10.1371/journal.pone.0003944. Epub 2008 Dec 15"
COMMENT     Sequence Label: mKikGR-C1
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..733
                     /codon_start=1
                     /product="monomeric photoconvertible Kikume green-to-red 
                     fluorescent protein"
                     /label=mKikGR1
                     /translation="MVSVITSEMKIELRMEGSVNGHKFVITGKGSGRPYEGTQTVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQLFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNTFVNEIRFDGTNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVEM
                     ALLLEGGGHYRCDFRTTYKAKKVVQLPDYHYVDHQMEITSHDKDYNKVKAYEHAKAYSG
                     TYRGAKYEFEA"
     misc_feature    734..799
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agtgtgatta catcagaaat
       61 gaagatcgag ctgcgtatgg aaggctctgt aaacgggcac aagttcgtga ttacagggaa
      121 aggaagtggc agaccttacg agggaacaca gactgtggac ctgacagtca tagagggcgg
      181 acctcttcct tttgctttcg atatcctgac aacagcattc cattacggca accgggtatt
      241 tgtcgaatac ccagaagaaa tagtagacta cttcaagcag ttgtttcctg agggttattc
      301 ttgggaacga agcatgagtt acgaagacgg gggaatttgc ctcgccacaa acaatataac
      361 gatgaagaaa gacggcagca acacttttgt caatgaaatt cgatttgatg gtacaaactt
      421 tcctgccaat ggtccagtta tgcagaggaa gaccgtcaaa tgggagccat ccactgagaa
      481 aatgtatgtg cgtgatggag tgctgaaggg tgatgttgaa atggctctgt tgcttgaagg
      541 aggtggccat taccgatgtg acttcagaac tacttacaaa gcaaagaagg ttgtccagtt
      601 gccagactat cactacgtgg atcatcaaat ggagataaca agccatgaca aggattacaa
      661 caaggttaag gcgtatgaac atgctaaggc ttattccggg acctacaggg gcgccaagta
      721 tgaatttgaa gcctccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg
      781 taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc ataccacatt
      841 tgtagaggtt ttacttgctt taaa
//
