LOCUS       sequence-89895-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mEos-A69V-N1
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     CDS             101..781
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFVEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atgagtgcga ttaagccaga
      121 catgaagatc aacctccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg
      181 agatggtaca ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg
      241 cggacctctg ccttttgcct ttgatatcct gaccactgca ttccattacg gcaacagggt
      301 attcgtcgaa tatccagacc acatacaaga ctattttaag cagtcgtttc ctaaggggta
      361 ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt tgcattgcca gaaacgacat
      421 aacaatggaa ggggacactt tctataataa agttcgattt cacggtacaa actttcccgc
      481 caatggtcca gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta
      541 tgtgcgtgat ggagtgctga cgggtgatat tcacatggct ttgttgcttg aaggaaatgc
      601 ccattaccga tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc
      661 aggctaccac tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa
      721 ggttaagctg tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgata
      781 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      841 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      901 ttaacttgtt tattgcagct tataatggtt acaaata
//
