LOCUS       sequence-89898-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 576, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AsRed2-N1
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..799
                     /codon_start=1
                     /product="Anemonia sulcata red fluorescent protein"
                     /label=AsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASLLKKTMPFRTTIEGTVNGHYFKCTGKGEGNPLEGTQEMKIEV
                     IEGGPLPFAFHILSTSCMYGSKAFIKYVSGIPDYFKQSLPEGFTWERTTTYEDGGFLTA
                     HQDTSLDGDCLVYKVKILGNNFPADGPVMQNKAGRWEPSTEIVYEVDGVLRGQSLMALE
                     CPGGRHLTCHLHTTYRSKKPASALKMPGFHFEDHRIEILEEVEKGKCYKQYEAAVGRYC
                     DAAPSKLGHN"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggcctctt tgctgaagaa
      121 gaccatgccc ttcaggacca ccatcgaggg caccgtgaac ggccactact tcaagtgcac
      181 cggcaagggc gagggcaacc ccctggaggg cacccaggag atgaagatcg aggtgatcga
      241 gggcggcccc ctgcccttcg ccttccacat cctgtccacc tcctgcatgt acggctccaa
      301 ggccttcatc aagtacgtgt ccggcatccc cgactacttc aagcagtccc tccccgaggg
      361 cttcacctgg gagcgcacca ccacctacga ggacggcggc ttcctgaccg cccaccagga
      421 cacctccctg gacggcgact gcctggtgta caaggtgaag atcctgggca acaacttccc
      481 cgccgacggc cccgtgatgc agaacaaggc cggccgctgg gagccctcca ccgagatcgt
      541 gtacgaggtg gacggcgtgc tgcgcggcca gtccctgatg gccctggagt gccccggcgg
      601 tcgccacctg acctgccacc tgcacaccac ctaccgctcc aagaagcccg cctccgccct
      661 gaagatgccc ggcttccact tcgaggacca ccgcatcgag atcctggagg aggtggagaa
      721 gggcaagtgc tacaagcagt acgaggccgc cgtgggccgc tactgcgacg ccgccccctc
      781 caagctgggc cacaactgaa gcggccgcga ctctagatca taatcagcca taccacattt
      841 gtagaggttt tacttgcttt aaa
//
