LOCUS       sequence-8990-ex        4809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, 
  TITLE     Single-copy insertion of transgenes in Caenorhabditis elegans
  PUBMED    18953339
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJL44 - Phsp16.48::MosTase::glh-2utr
FEATURES             Location/Qualifiers
     source          1..4809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        95..401
                     /label=hsp16-48 promoter
                     /note="promoter for the C. elegans heat shock protein 16-48
                     gene (Russnak and Candido, 1985)"
     primer_bind     complement(2383..2405)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2505..2524
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2602..2620)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2688..2792
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2793..2861,2862..3653)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3011..3030)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3824..4412
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4313..4332
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4566..4583
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4700..4721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4736..4741,4742..4759,4760..4766)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4774..4790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4779..4801
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(4798..4809,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(4798..4809,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgctggac ggaaatagtg gtaaagtgac atgattgtag
       61 tttgaagatt tcacaattag agtgaatgtt gtttggttcg gttttgtcac tgtatttata
      121 ctcatttcca cctttttcta gaacattcga gctgcttctt gcaaaaggag ggcgactcac
      181 attcagaaca ttgagaaata gtgtgcgtac tgaagaaacc cagatacttt ttcaatctgc
      241 gtctctttgc acctatgggg tgtattttga aatgaatgca tctaggacct tctagaacat
      301 tctaaacggc tgcaggatac gggtatataa gccaatcgtg ttcagaggaa accaatacac
      361 tttgttcaag tgcttactgt tcattctcta aacttcaaga acgcgttatg tcgagtttcg
      421 tgccgaataa agagcaaacg cggacagtat taattttctg ttttcatttg aagaaaacag
      481 ctgcggaatc gcaccgaatg cttgttgaag cctttggcga acaagtacca actgtgaaaa
      541 cgtgtgaacg gtggtttcaa cgcttcaaaa gtggtgattt tgacgtcgac gacaaagagc
      601 acggaaaacc gccaaaaagg tacgaagacg ccgaactgca agcattattg gatgaagacg
      661 atgctcaaac gtaagtttaa acatatatac taactaaccc atggattatt taaattttca
      721 ggcaaaaaca actcgcagag cagttggaag taagtcaaca agcagtttcc aatcgcttgc
      781 gagagatggg aaagattcag aaggtcggta gatgggtgcc acatgagttg aacgagaggc
      841 agatggagag gcgcaaaaac acatgcgaaa ttttgctttc acgatacaaa aggaagtcgt
      901 ttttgcatcg tatcgttact ggagatgaaa aatggatctt ttttgttaat cctaaacgta
      961 aaaagtcata cgttgatcct ggacaaccgg ccacatcgac tgctcgaccg aatcgctttg
     1021 gcaagaagac gatgctctgt gtttggtggg atcagagcgg tgtcatttac tatgagctct
     1081 tgaaacccgg cgaaacggtg aatacggcac gctaccaaca acaattgatc aatttgaacc
     1141 gtgcgcttca gagaaaacga ccggaatatc aaaaaagaca acacagggtc atttttctcc
     1201 atgacaacgc tccatcacat acggcaagag cggttcgcga cacgttggaa acactcaatt
     1261 gggaagtgct tccgcatgcg gcttactcac cagacctggc cccatccgat taccacctat
     1321 tcgcttcgat gggacacgca ctcgctgagc agcgcttcga ttcttacgaa agtgtgaaaa
     1381 aatggctcga tgaatggttc gccgcaaaag acgatgagtt ctactggcgt ggaatccaca
     1441 aattgcccga gagatgggaa aaatgtgtcg cgagcgacgg caaatacttt gaatagctag
     1501 catgtatctg catatccttc aaatgttgtc catattgtat cagtaaatta taatgccccc
     1561 tttttatata ttctccttat catgtaaatg ttctattgat tttttgctgg tgtaaaacgt
     1621 ttttatagta aatcctctcc acagtgaatt tgttaaattg tatcaaaaca agacattgat
     1681 taaatttgtt cgagacttgt ggattgtaaa atattttgca gaaaatggca atattcattt
     1741 agaaacatta cttccataat aggaaaaact atttatgagt tttcatgtgc ctatgcaagt
     1801 tcccactctg tgtgaatttc atcatacaaa ttgagcaaac ataaggttta tgtccagtat
     1861 gggttctcag atgttttgta agagttgagc tgtcggtgaa tgccttctca cattgagcac
     1921 atggatacgg tctctctcca gtgtgtgttc tgaaataatg aaaaatagtc ttgcaatatg
     1981 tttctactat tcgagaaaaa atgtaatttt ttttcttatg gtaacctgca atttatatat
     2041 tttttatttg ccgttattga cattattata agaaattgga ggatatcaca aatatcgtaa
     2101 attctccaaa attttgacac ttcactttaa agacaaatgc ggtttttccg agtgtgtctc
     2161 gccacgatct cagtcataat ggtcgctaca ggtgccaaac tttaggcatt tgttatattt
     2221 gatgcagaaa accaagcaca tgaaaattaa aattgtcaag tagacaatga aatccagaca
     2281 ttgttatgat tcattttttt gcgaattcct gcagcccggg ggatccacta gtcggccgta
     2341 cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2401 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2461 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2521 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2581 ccgcatcagg cggccttaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     2641 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2701 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2761 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2821 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2881 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2941 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3001 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3061 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3121 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3181 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3241 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3301 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3361 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3421 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3481 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3541 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3601 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3661 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3721 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3781 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3841 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3901 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3961 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4021 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4081 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4141 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4201 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4261 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4321 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4381 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4441 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4501 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4561 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4621 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4681 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     4741 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     4801 gaaacagct
//
