LOCUS       sequence-89900-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 440, Emission: 620.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKeima-Red-N1
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(101..103,104..106,107..769)
                     /codon_start=1
                     /product="monomeric Keima-Red fluorescent protein, also 
                     known as mKeima"
                     /label=mKeima-Red
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagtg tgatcgctaa
      121 acaaatgacc tacaaggttt atatgtcagg cacggtcaat ggacactact ttgaggtcga
      181 aggcgatgga aaaggaaagc cttacgaggg agagcagaca gtaaagctca ctgtcaccaa
      241 gggtggacct ctgccatttg cttgggatat tttatcacca cagcttcagt acggaagcat
      301 accattcacc aagtaccctg aagacatccc tgattatttc aagcagtcat tccctgaggg
      361 atatacatgg gagaggagca tgaactttga agatggtgca gtgtgtactg tcagcaatga
      421 ttccagcatc caaggcaact gtttcatcta caatgtcaaa atctctggtg agaactttcc
      481 tcccaatgga cctgttatgc agaagaagac acagggctgg gaacccagca ctgagcgtct
      541 ctttgcacga gatggaatgc tgataggaaa cgattatatg gctctgaagt tggaaggagg
      601 tggtcactat ttgtgtgaat ttaaatctac ttacaaggca aagaagcctg tgaggatgcc
      661 agggcgccac gagattgacc gcaaactgga tgtaaccagt cacaacaggg attacacatc
      721 tgttgagcag tgtgaaatag ccattgcacg ccactctttg ctcggttaag cggccgcgac
      781 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aa
//
