LOCUS       sequence-89901-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 475, Emission: 505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AcGFP1-N1
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..93
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..820
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgccga
      121 gctgttcacc ggcatcgtgc ccatcctgat cgagctgaat ggcgatgtga atggccacaa
      181 gttcagcgtg agcggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      241 catctgcacc accggcaagc tgcctgtgcc ctggcccacc ctggtgacca ccctgagcta
      301 cggcgtgcag tgcttctcac gctaccccga tcacatgaag cagcacgact tcttcaagag
      361 cgccatgcct gagggctaca tccaggagcg caccatcttc ttcgaggatg acggcaacta
      421 caagtcgcgc gccgaggtga agttcgaggg cgataccctg gtgaatcgca tcgagctgac
      481 cggcaccgat ttcaaggagg atggcaacat cctgggcaat aagatggagt acaactacaa
      541 cgcccacaat gtgtacatca tgaccgacaa ggccaagaat ggcatcaagg tgaacttcaa
      601 gatccgccac aacatcgagg atggcagcgt gcagctggcc gaccactacc agcagaatac
      661 ccccatcggc gatggccctg tgctgctgcc cgataaccac tacctgtcca cccagagcgc
      721 cctgtccaag gaccccaacg agaagcgcga tcacatgatc tacttcggct tcgtgaccgc
      781 cgccgccatc acccacggca tggatgagct gtacaagtaa gcggccgcga ctctagatca
      841 taatcagcca taccacattt gtagaggtt
//
