LOCUS       sequence-89905-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 492, Emission: 505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Karasawa S, Araki T, Yamamoto-Hino M, Miyawaki A
  TITLE     A green-emitting fluorescent protein from Galaxeidae coral and its 
            monomeric version for use in fluorescent labeling.
  JOURNAL   J Biol Chem. 2003 Sep 5. 278(36):34167-71.
  PUBMED    12819206
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Sep 5. 278(36):34167-71."
COMMENT     Sequence Label: mAzami Green-C1 (mAG)
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..712
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             35..712
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     misc_feature    713..778
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcgtgatca agcccgagat
       61 gaagatcaag ctgtgcatga ggggcaccgt gaacggccac aacttcgtga tcgagggcga
      121 gggcaagggc aacccctacg agggcaccca gatcctggac ctgaacgtga ccgagggcgc
      181 ccccctgccc ttcgcctacg acatcctgac caccgtgttc cagtacggca acagggcctt
      241 caccaagtac cccgccgaca tccaggacta cttcaagcag accttccccg agggctacca
      301 ctgggagagg agcatgacct acgaggacca gggcatctgc accgccacca gcaacatcag
      361 catgaggggc gactgcttct tctacgacat caggttcgac ggcaccaact tcccccccaa
      421 cggccccgtg atgcagaaga agaccctgaa gtgggagccc agcaccgaga agatgtacgt
      481 ggaggacggc gtgctgaagg gcgacgtgaa catgaggctg ctgctggagg gcggcggcca
      541 ctacaggtgc gacttcaaga ccacctacaa ggccaagaag gaggtgaggc tgcccgacgc
      601 ccacaagatc gaccacagga tcgagatcct gaagcacgac aaggactaca acaaggtgaa
      661 gctgtacgag aacgccgtgg ccaggtactc catgctgccc agccaggcca agtccggact
      721 cagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac
      781 cggatctaga taactgatca taatcagcca taccacattt gtagaggtt
//
