LOCUS       sequence-89908-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Synthetic promoter responsive to gRNA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK
  TITLE     Multiplexed and Programmable Regulation of Gene Networks with an 
            Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
  JOURNAL   Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022.
  PUBMED    24837679
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022."
COMMENT     Sequence Label: P1-EYFP-pA (Construct 5)
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..700)
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGDVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEIAGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLTE
                     TVRAVDCRKTY"
ORIGIN
        1 ggtaggtctt gcggcaatcc acggcgcgca cggtctccgt caggctcata ttatcgcggt
       61 gatcggtcac atccttgctc agcttcacgt ggtagctcat gtggtggtac tcgggcacgt
      121 gcacggggcc gccgccgatg ggggtattca tctgggtgtg atcggccacg atgaaatcgc
      181 ccttgctgcc ggcgatctcg tggcagatct tgaaggcgct cttcaggccg tgattggcct
      241 gatcgcccca gatgtacagg cagtgggggg tgaaattgaa ctccagattc ttgcccagca
      301 cgtggccatc cttcttgaag ccctggccat tcagcttcac gcgattgtac acgctgccat
      361 tctcgaaggt cacctcggcg cgggtcttga aattgccatc gccctcgaag gtgatggtgc
      421 gctcctgcac gtagccatcg ggcatgcagc tcttgtagaa atccttcagc tcggggccgt
      481 acttggcgaa gcactgggcg ccgtaggtca gggtggtcac cagggtgctc cagggcacgg
      541 gcacatcgcc ggtggtgcag atgaactggg catccacctt gcccacgctg gcatcgccgt
      601 agcccttacc gcggatgctg aaggtgtggc catccacatc gccctccatc tccaccacgt
      661 aggggatctt gccgtggaac agcagggcgc cgctgctcat ggtggcgcca tggtcaacgc
      721 gacgctagat agcaggacga acgcccccac ccccttttat agccccccgc tagcctgaaa
      781 atattaatag tcgcgtgtag cgaagcatgg ctagcctg
//
