LOCUS       sequence-89910-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses taCas9 in Mammalian cells for transactivating endogenous 
            and synthetic promoters. The backbone is a lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK
  TITLE     Multiplexed and Programmable Regulation of Gene Networks with an 
            Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
  JOURNAL   Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022.
  PUBMED    24837679
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022."
COMMENT     Sequence Label: CMVp-dCas9-3xNLS-VP64 (Construct 1)
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..195)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(235..255)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(262..282)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(289..309)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttgtaatcca gaggttgatt atcgataagc ttgatatcgt catgagagca tatccaggtc
       61 aaaatcatcc aaggcatctg acccgagcat gtcgaggtcg aaatcgtcga gagcgtcgct
      121 gccgagcata tccagatcga aatcatccag ggcgtcagaa ccgagcatat cgagatcgaa
      181 atcgtccaaa gcgtctgaac ctccaccgcc gttgctgcct gagccgatcc catccacctt
      241 cctctttttc ttggggtctt caaccttcct tttcttcttt ggatcctcga ctttgcgttt
      301 cttcttgggt gaccctgaac ccagtgaggg cccgtcagcc ctgctgtctc caccgagctg
      361 agagaggtcg attcttgttt catagagccc cgtaattgac tgatgaatca gtgtggcgtc
      421 caggacctcc tttgtagagg tgtaccgctt tctgtctatg gtggtgtcga agtacttgaa
      481 ggctgcaggc gcgcccaagt tggtcagagt aaacaagtgg ataatgtttt ctgcctgctc
      541 cctgatgggc ttatccctgt gcttattgta agcagaaagc accttatcga ggttagcgtc
      601 ggcgaggatc actcttttgg agaattcgct tatttgctcg atgatctcat caaggtagtg
      661 tttgtgttgt tccacgaaca gctgcttctg ctcattatct tcgggagacc ctttgagctt
      721 ttcatagtgg ctggccagat acaagaaatt aacgtattta gagggcagtg ccagctcgtt
      781 acctttctgc agctcgcccg cactagcgag cattcgtttc cggccgtttt caagctcaaa
      841 gagagagtac ttgggaagct taatgatgag gtctt
//
