LOCUS       sequence-89913-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac His6 TEV cloning vector with BioBrick...
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     mobile_element  251..416
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    490..624
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(514..533)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     568..587
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(869..889)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(890..907)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
       61 ggcgcgtccc attcgccatt caggctgcaa ataagcgttg atattcagtc aattacaaac
      121 attaataacg aagagatgac agaaaaattt tcattctgtg acagagaaaa agtagccgaa
      181 gatgacggtt tgtcacatgg agttggcagg atgtttgatt aaaaacataa caggaagaaa
      241 aatgccccgc tgtgggcgga caaaatagtt gggaactggg aggggtggaa atggagtttt
      301 taaggattat ttagggaaga gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct
      361 aatacgaaaa ttaaaaatga caaaatagtt tggaactaga tttcacttat ctggttcgga
      421 tctcccctgc aggcgtttaa acctaactcc atttaaatgg tggatgtttc taggctcaag
      481 cagtgatcag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
      541 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      601 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      661 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      721 gattatgatc ctctagtact tctcgacaag cttggtaccg catgcctcga gtgcggcccc
      781 taggagatca tgaggcccct aggagatcat gaggccgcaa gcttgtcgac ggcgctcgaa
      841 ttcggatccg ttatccactt ccaatattgg attggaagta caggttttca tggtgatggt
      901 gatggtgaga agaacccatc ggaccgggat ccgcgcccga tggtgggacg gtatgaataa
      961 tccggaatag ttataggttt ttttattaca aaactgttac
//
