LOCUS       sequence-89915-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses taCas9 in Mammalian cells for transactivating endogenous 
            and synthetic promoters. The backbone is a lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK
  TITLE     Multiplexed and Programmable Regulation of Gene Networks with an 
            Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
  JOURNAL   Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022.
  PUBMED    24837679
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022."
COMMENT     Sequence Label: CMVp-dCas9-3xNLS-VP64 (Construct 1)
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      284..293
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(440..459)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 gagtcatcca ccttagccat ctcattacta aagatctcct gcaggtagca gatccgattc
       61 tttctgcggg tatatctgcg ccgtgctgtt cttttgagcc gcgtggcttc ggccgtctcc
      121 ccggagtcga acaggagggc gccaatgagg ttcttcttta tgctgtggcg atcggtattg
      181 cccagaactt tgaatttttt gctcggcacc ttgtactcgt ccgtaatgac ggcccagccg
      241 acgctgtttg tgccgatggc gagcccaatg gagtacttct tgtccatggt ggcctgtcta
      301 acaaaaaagc caaaaacggc cagaatttag cggacaattt actagtctaa cactgaaaat
      361 tacatattga cccaaatgat tacatttcaa aaggtgccta aaaaacttca caaaacacac
      421 tcgccaaccc cgagcgcata gttcaaaacc ggagcttcag ctacttaaga agataggtac
      481 ataaaaccga ccaaagaaac tgacgcctca cttatccctc ccctcaccag aggtccggcg
      541 cctgtcgatt caggagagcc taccctaggc ccgaaccctg cgtcctgcga cggagaaaag
      601 cctaccgcac acctaccggc aggtggcccc accctgcatt ataagccaac agaacgggtg
      661 acgtcacgac acgacgaggg cgcgcgctcc caaaggtacg ggtgcactgc ccaa
//
