LOCUS       sequence-89916-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses taCas9 in Mammalian cells for transactivating endogenous 
            and synthetic promoters. The backbone is a lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK
  TITLE     Multiplexed and Programmable Regulation of Gene Networks with an 
            Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
  JOURNAL   Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022.
  PUBMED    24837679
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022."
COMMENT     Sequence Label: CMVp-dCas9-3xNLS-VP64 (Construct 1)
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(90..101)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cttccgttgt gtgatcagtt tggcgttcag cagctgccgc caataatttt tcattttctt
       61 gacaacttct tctgagggga cgttatcact cttccctcta tttttatcgg atcttgtcaa
      121 cactttatta tcaatagaat catctttgag aaaagactgg ggcacgatgg catccacgtc
      181 gtagtcggag agccgattga tgtccagttc ctgatccacg tacatgtccc tgccgttctg
      241 caggtagtac aggtagagct tctcattctg aagctgggtg ttttcaactg ggtgttcctt
      301 aaggatttgg gaccccagtt cttttatacc ctcttcaatc ctcttcatcc tttccctact
      361 gttcttctgt cccttctggg tagtttggtt ctctcgggcc atctcgataa cgatattctc
      421 gggcttatgc cttcccatta ctttgacgag ttcatccacg accttaacgg tctgcagtat
      481 tccctttttg atagctgggc tacctgcaag attagcgatg tgctcgtgaa gactgtcccc
      541 ctggccagaa acttgtgctt tctggatgtc ctccttaaag gtgagagagt catcatggat
      601 caactgcatg aagttccggt tggcaaatcc atcggactta agaaaatcca ggattgtctt
      661 tccactctgc ttgtctcgga tcccattgat cagttttctt gacagccgcc cccatcctgt
      721 atatcggcgc ctcttgagct gtttcatgac tttgtcgtcg aagagatgag cgtaagtttt
      781 caagcgttct tcaatcatct ccctatcttc aaacaacgta agggtgagg
//
