LOCUS       sequence-89917-e        1114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac TEV SNAPf Prescission TwinStrepII clo...
FEATURES             Location/Qualifiers
     source          1..1114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(190..195,196..212,213..218))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     627..644
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
       61 gcccgaggca tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
      121 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
      181 ttgcattaca gtttacgaac cgaacaggct tatgtcaact gggttcgtgc cttcatccgt
      241 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
      301 tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg
      361 ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga
      421 cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc
      481 ggttttctgg aaggcgagca tcgtttgttc gcccaggact ctagctatag ttctagtggt
      541 tggctacgta tactccggac tattaataga tcatggagat aattagcggc cgcgtttaaa
      601 catccaccat ttgatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt
      661 tactgttttc gtaacagttt tgtaataaaa aaacctataa ctattccgga ttattcatac
      721 cgtcccacca tcgggcgcgg atccctttaa gaaggagata tagttaacct ctacttccaa
      781 tccggctctg ataaagactg cgagatgaag cgcacgacgc tcgattcgcc ccttggcaaa
      841 ttggaactga gcggttgtga gcaaggtctg catcgtatca tctttctggg aaagggaact
      901 agtgcagcag atgcagtcga ggtcccggct cctgctgccg tgcttggtgg tcccgagcct
      961 ttgatgcaag ctaccgcttg gctgaatgca tatttccatc agccagaggc gatcgaggag
     1021 ttccccgttc ccgcgctcca tcatccggtt ttccaacagg aatccttcac ccgccaggtc
     1081 ctgtggaagc tcctgaaggt cgtaaagttt ggtg
//
