LOCUS       sequence-89942-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes module 4 with amino acids 12C-16Q for recognition of nt 5A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pChlr-4CQ
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(155..171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(155..171)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(168..190)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    179..195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(203..209,210..227,228..233))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    248..269
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(386..403)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(637..656)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagctcggtc tcatggccat gtcttgaagt gtgtgaaaga tcagaatggc tgtcacgtgg
       61 ttcagaaatg cattgaatgt gtacagcccc agtctttgca atttatcatc gatgcgttta
      121 agggacagga gagaccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      181 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      241 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      301 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      361 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      421 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      481 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      541 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      601 gctcaagtca gaggtggtga aacccgacag gactataaag ataccaggcg tttccccctg
      661 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      721 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgta
//
