LOCUS       sequence-89943-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes module 4 with amino acids 12S-16E for recognition of nt 5G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pChlr-4SE
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(154..170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(154..170)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(167..189)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    178..194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(202..208,209..226,227..232))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    247..268
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(385..402)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(636..655)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agctcggtct catggccatg tcttgaagtg tgtgaaagat cagaatggca gtcacgtggt
       61 tgagaaatgc attgaatgtg tacagcccca gtctttgcaa tttatcatcg atgcgtttaa
      121 gggacaggag agaccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
      181 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
      241 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
      301 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      361 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      421 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      481 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      541 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      601 ctcaagtcag aggtggtgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      661 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      721 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgta
//
