LOCUS       sequence-89944-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes module 4 with amino acids 12N-16Q for recognition of nt 5U.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pChlr-4NQ
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(157..173)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(157..173)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(170..192)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(205..211,212..229,230..235))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    250..271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(388..405)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(639..658)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgagctcgg tctcatggcc atgtcttgaa gtgtgtgaaa gatcagaatg gcaatcacgt
       61 ggttcagaaa tgcattgaat gtgtacagcc ccagtctttg caatttatca tcgatgcgtt
      121 taagggacag gagagaccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
      181 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
      241 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
      301 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      361 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      421 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      481 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      541 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      601 acgctcaagt cagaggtggt gaaacccgac aggactataa agataccagg cgtttccccc
      661 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      721 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      781 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa cc
//
