LOCUS       sequence-89945-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes module 4 with amino acids 12S-16R for recognition of nt 5C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pChlr-4SR
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(156..172)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(156..172)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(169..191)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(204..210,211..228,229..234))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    249..270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(387..404)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(638..657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgagctcggt ctcatggcca tgtcttgaag tgtgtgaaag atcagaatgg cagtcacgtg
       61 gttcgcaaat gcattgaatg tgtacagccc cagtctttgc aatttatcat cgatgcgttt
      121 aagggacagg agagaccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      181 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      241 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      301 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      361 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      421 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      481 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      541 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      601 cgctcaagtc agaggtggtg aaacccgaca ggactataaa gataccaggc gtttccccct
      661 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      721 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      781 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cc
//
