LOCUS       sequence-89951-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes module 5 with amino acids 12S-16E for recognition of nt 4G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pChlr-5SE
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(153..169)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(153..169)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(166..188)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    177..193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(201..207,208..225,226..231))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    246..267
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(384..401)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(635..654)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgagctcgg tctcacaggt atttgcctta tccacacatc cttatggctc ccgagtgatt
       61 gagagaatcc tggagcactg tctccctgac cagacactcc ctattttaga ggagcttcac
      121 cagcacaaga gaccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      181 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      241 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      301 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      361 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      421 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      481 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      541 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      601 tcaagtcaga ggtggtgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      661 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      721 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      781 taggtcgttc gctccaagct gggctgtgtg cacgaaccc
//
