LOCUS       sequence-89953-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes module 5 with amino acids 12S-16R for recognition of nt 4C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pChlr-5SR
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(150..166)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(150..166)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(163..185)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    174..190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(198..204,205..222,223..228))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    243..264
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(381..398)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(632..651)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agctcggtct cacaggtatt tgccttatcc acacatcctt atggctcccg agtgattcgc
       61 agaatcctgg agcactgtct ccctgaccag acactcccta ttttagagga gcttcaccag
      121 cacaagagac caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
      181 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      241 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      301 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      361 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      421 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      481 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      541 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      601 agtcagaggt ggtgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      661 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      721 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      781 gtcgttcgct ccaagctggg ctgtgtgcac gaaccc
//
