LOCUS       sequence-89958-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes module 7 with amino acids 12C-16Q for recognition of nt 2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pChlr-7CQ
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(159..175)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(159..175)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(172..194)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    183..199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(207..213,214..231,232..237))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    252..273
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(390..407)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(641..660)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatgtacttg tattgagtca gcacaaattt gcatgcaatg ttgtgcagaa gtgtgttact
       61 cacgcctcac gtacggagcg cgctgtgctc atcgatgagg tgtgcaccat gaacgacggt
      121 ccccacagtg cctagagacc aagcttggcg taatcatggt catagctgtt tcctgtgtga
      181 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      241 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      301 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
      361 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
      421 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      481 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      541 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      601 cgacgctcaa gtcagaggtg gtgaaacccg acaggactat aaagatacca ggcgtttccc
      661 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      721 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      781 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccc
//
