LOCUS       sequence-89963-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes module 8 with amino acids 12S-16E for recognition of nt 1G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pChlr-8SE
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(147..163)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(147..163)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(160..182)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    171..187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(195..201,202..219,220..225))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    240..261
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(378..395)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(629..648)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagctcggtc tcagccttat acaccatgat gaaggaccag tatgccagct acgtggtcga
       61 gaagatgatt gacgtggcgg agccaggcca gcggaagatc gtcatgcata agatccgacc
      121 cagagaccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      181 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      241 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      301 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      361 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      421 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      481 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      541 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      601 cagaggtggt gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      661 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      721 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      781 gttcgctcca agctgggctg tgtgcacgaa cc
//
