LOCUS       sequence-89966-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Receiving vector for PUM-HD assembly and expression in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Abil Z, Denard CA, Zhao H
  TITLE     Modular assembly of designer PUF proteins for specific 
            post-transcriptional regulation of endogenous RNA.
  JOURNAL   J Biol Eng. 2014 Mar 1;8(1):7.
  PUBMED    24581042
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2014-03-1"; volume: "8(1)"; pages: "7"
COMMENT     Sequence Label: pET28-GG-PUF
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(77..93)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(77..93)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(90..112)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    101..117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(125..131,132..149,150..155))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    170..191
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             350..367
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(420..438)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      434..481
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(605..624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     815..836
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggggcgcgcc ggatccttaa ttaagtctag agtcgactgt ttaaacctgc aggcatgcaa
       61 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      121 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      181 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcggtctca accccacatc
      241 gcaactcttc gtaagtacac ctatggcaag cacattctgg ccaagctgga gaagtactac
      301 atgaagaacg gtgttgactt agggtaacaa gcttgcggcc gcactcgagc accaccacca
      361 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      421 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      481 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      541 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      601 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      661 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      721 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      781 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
      841 acactcaacc ctatctcggt ctattctt
//
