LOCUS       sequence-89971-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 580, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKind1-N1
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..803
                     /codon_start=1
                     /product="FusionRed fluorescent protein derivative that 
                     shows reversible photoswitching with visible light and fast
                     OFF-switching kinetics"
                     /label=rsFusionRed2
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSKTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEAHTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcgagctga
      121 ttaaggagaa catgcccatg aagctgtaca tggagggcac cgtgaacaac caccacttca
      181 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg
      241 tcgtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg
      301 gcagcaaaac cttcatcaag caccctccgg gcatccccga cttctttaag cagtccttcc
      361 ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta
      421 cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaaggtt agaggggtga
      481 acttcccagc caacggccct gtgatgcaga agaaaacact cggctgggag gcccacaccg
      541 agacgatgta ccccgctgac ggcggcctgg aaggcgcatg tgacatggcc ctgaagctcg
      601 tgggcggggg ccacctgatc tgcaaccttg agaccacata cagatccaag aaacccgcta
      661 cgaacctcaa gatgcccggc gtctacaacg tggaccacag actggaaaga atcaaggagg
      721 ccgacgatga gacctacgtc gagcagcacg aggtggctgt ggccagatac tctactggtg
      781 gcgctggtga tggaggtaaa tgagcggccg cgactctaga tcataatcag ccataccaca
      841 tttgtagagg ttttacttgc tt
//
