LOCUS       sequence-89975-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Synthetic promoter responsive to gRNA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK
  TITLE     Multiplexed and Programmable Regulation of Gene Networks with an 
            Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
  JOURNAL   Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022.
  PUBMED    24837679
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022."
COMMENT     Sequence Label: P2-ECFP-pA (Construct 6)
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    138..229
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     178..197
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     primer_bind     complement(671..692)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag gtacgggagg
       61 tacttggagc ggccgcaata aatatcttta ttttcattac atctgtgtgt tggttttttg
      121 tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta
      181 gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgatagcta
      241 gcccaggaca gtactccgac ttacttaata ttttcaggct agcccaggac agtactccga
      301 cttacttaat attttcaggc tagcccagga cagtactccg acttacttaa tattttcagg
      361 ctagcccagg acagtactcc gacttactta atattttcag gctagcccag gacagtactc
      421 cgacttactt aatattttca ggctagccca ggacagtact ccgacttact taatattttc
      481 aggctagccc aggacagtac tccgactaac ttaatatttt caggctagcc caggacagta
      541 ctccgactta ctaatatttt cagctagcgg ggggctataa aagggggtgg gggcgttcgt
      601 cctgctatct agcgtcgcgt tgaccatggt gagcaagggc gaggagctgt tcaccggggt
      661 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      721 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      781 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt
      841 cgcccgctac cccgaccaca tgaagcagca c
//
