LOCUS       sequence-89976-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 588, Emission: 618.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: HcRed1-N1
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    27..107
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      109..118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             115..801
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
ORIGIN
        1 agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag
       61 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg
      121 agcggcctgc tgaaggagag tatgcgcatc aagatgtaca tggagggcac cgtgaacggc
      181 cactacttca agtgcgaggg cgagggcgac ggcaacccct tcgccggcac ccagagcatg
      241 agaatccacg tgaccgaggg cgcccccctg cccttcgcct tcgacatcct ggccccctgc
      301 tgcgagtacg gcagcaggac cttcgtgcac cacaccgccg agatccccga cttcttcaag
      361 cagagcttcc ccgagggctt cacctgggag agaaccacca cctacgagga cggcggcatc
      421 ctgaccgccc accaggacac cagcctggag ggcaactgcc tgatctacaa ggtgaaggtg
      481 cacggcacca acttccccgc cgacggcccc gtgatgaaga acaagagcgg cggctgggag
      541 cccagcaccg aggtggtgta ccccgagaac ggcgtgctgt gcggccggaa cgtgatggcc
      601 ctgaaggtgg gcgaccggca cctgatctgc caccactaca ccagctaccg gagcaagaag
      661 gccgtgcgcg ccctgaccat gcccggcttc cacttcaccg acatccggct ccagatgctg
      721 cggaagaaga aggacgagta cttcgagctg tacgaggcca gcgtggcccg gtacagcgac
      781 ctgcccgaga aggccaacta agcggccgcg actctagatc ataatcagcc ataccacatt
      841 tgtagaggtt
//
