LOCUS       sequence-89980-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Synthetic promoter responsive to gRNA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK
  TITLE     Multiplexed and Programmable Regulation of Gene Networks with an 
            Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
  JOURNAL   Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022.
  PUBMED    24837679
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022."
COMMENT     Sequence Label: P1-EYFP-pA (Construct 5)
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    76..124
                     /note="synthetic polyadenylation signal"
     misc_feature    138..229
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     178..197
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ttcgctatta cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt
       61 acttggagcg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
      121 tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta
      181 gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgatagcta
      241 gctgaaatat taatgtcgcg actattaata ttttcaggct agccatgctt cgctacacgc
      301 gactattaat attttcaggc tagccatgct tcgctacacg cgactattaa tattttcaag
      361 ctagccatgc ttcgctacac gcgactatta atattttcag gctagccatg cttcgctaac
      421 gcgactatta atattttcag gctagtcatg cttcgctaca cgcgactatt aatattttca
      481 ggctagccat gcttcgctac acgcgactat taatattttc aggctagcca tgcttcgcta
      541 cacgcgacta ttaatatttt caggctagcc atgcttcgct acacgcgact attaatattt
      601 tcaggctagc ggggggctat aaaagggggt gggggcgttc gtcctgctat ctagcgtcgc
      661 gttgaccatg gcgccaccat gagcagcggc gccctgctgt tccacggcaa gatcccctac
      721 gtggtggaga tggagggcga tgtggatggc cacaccttca gcatccgcgg taagggctac
      781 ggcgatgcca gcgtgggcaa ggtggatgcc cagttcatct gcaccaccgg cgatgtgccc
      841 gtgccctgga gcaccctggt gaccaccctg acctacg
//
