LOCUS       sequence-89982-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 507 / 583, Emission: 
            517 / 593.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: wtKikGR-C1
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..723
                     /codon_start=1
                     /product="photoconvertible Kikume green-to-red fluorescent 
                     protein (Tsutsui et al., 2005)"
                     /label=KikGR1
                     /translation="MVSVITSEMKIELRMEGAVNGHKFVITGKGSGQPFEGIQNVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQSFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNCFVNEIRFDGVNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVNM
                     ALLLQGGGHYRCDFRTTYKAKKVVQLPDYHFVDHQMEITSHDKDYNKVKLYEHAKAHSG
                     LPRLAK"
     misc_feature    724..789
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgagtgt gattacatca
       61 gaaatgaaga tcgagctgcg tatggaaggc gctgtaaacg ggcacaagtt cgtgattaca
      121 gggaaaggaa gtggccagcc tttcgaggga atacagaatg tggacctgac agtcatagag
      181 ggcggacctc ttccttttgc tttcgatatc ctgacaacag cattccatta cggcaaccgg
      241 gtatttgtcg aatacccaga agaaatagta gactacttca agcagtcgtt tcctgagggt
      301 tattcttggg aacgaagcat gagttacgaa gacgggggaa tttgcctcgc cacaaacaat
      361 ataacgatga agaaagacgg cagcaactgt tttgtcaatg aaattcgatt tgatggtgtg
      421 aactttcctg ccaatggtcc agttatgcag aggaagaccg tcaaatggga gccatccact
      481 gagaaaatgt atgtgcgtga tggagtgctg aagggtgatg taaacatggc tctgttgctt
      541 caaggaggtg gccattaccg atgtgacttc agaactactt acaaagcaaa gaaggttgtc
      601 cagttgccag actatcactt cgtggatcat caaatggaga taacaagcca tgacaaggat
      661 tacaacaagg ttaagctgta tgagcacgct aaagctcatt ccgggctgcc aaggctggcc
      721 aagtccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
      781 ccgggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggt
//
