LOCUS       sequence-89985-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses taCas9 in Mammalian cells for transactivating endogenous 
            and synthetic promoters. The backbone is a lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK
  TITLE     Multiplexed and Programmable Regulation of Gene Networks with an 
            Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
  JOURNAL   Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022.
  PUBMED    24837679
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022."
COMMENT     Sequence Label: CMVp-dCas9-3xNLS-VP64 (Construct 1)
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg
       61 tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc
      121 cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg
      181 gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag
      241 tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta
      301 tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg
      361 ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg
      421 gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg
      481 gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca
      541 aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg
      601 ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta
      661 atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt
      721 gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg
      781 cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac
      841 gtcacccgtt ctgttggctt ataatg
//
