LOCUS       sequence-89989-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding the Triplex/gRNA architecture.This is a modified 
            form of the original plasmid described in the paper (Construct 3). 
            mKate2 was replaced with dsRed2 because of distribution issues..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK
  TITLE     Multiplexed and Programmable Regulation of Gene Networks with an 
            Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
  JOURNAL   Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022.
  PUBMED    24837679
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022."
COMMENT     Sequence Label: CMVp-dsRed2-Triplex-28-gRNA1-28-pA
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             24..701
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(204..224)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     616..639
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ccgtcagatc cgagctcgcc accatggcct cctccgagaa cgtcatcacc gagttcatgc
       61 gcttcaaggt gcgcatggag ggcaccgtga acggccacga gttcgagatc gagggcgagg
      121 gcgagggccg cccctacgag ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc
      181 ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggtgtacg
      241 tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag ggcttcaagt
      301 gggagcgcgt gatgaacttc gaggacggcg gcgtggcgac cgtgacccag gactcctccc
      361 tgcaggacgg ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc ccctccgacg
      421 gccccgtgat gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc
      481 gcgacggcgt gctgaagggc gagacccaca aggccctgaa gctgaaggac ggcggccact
      541 acctggtgga gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg cccggctact
      601 actacgtgga cgccaagctg gacatcacct cccacaacga ggactacacc atcgtggagc
      661 agtacgagcg caccgagggc cgccaccacc tgttcctgta gtaaaccggt gattcgtcag
      721 tagggttgta aaggtttttc ttttcctgag aaaacaacct tttgttttct caggttttgc
      781 tttttggcct ttccctagct ttaaaaaaaa
//
