LOCUS       sequence-89991-e        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 High pI protein 69 TEV LIC cloning ve...
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     201..220
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     304..323
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        304..322
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             411..433
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             453..470
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             972..983
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1029..1049
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
       61 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
      121 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
      181 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
      241 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
      301 aattaatacg actcactata gggagaccac aacggtttcc ctctagtgcc ggctccggag
      361 agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat tttgtttaac
      421 tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat ggttcttcta
      481 tgaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc gaaattcgcg
      541 cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg agtctgagag
      601 aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc cctaacgccg
      661 agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag agcaagtctt
      721 ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa ttccgtcctg
      781 gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt ctcgaagagg
      841 gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac cagcaaatcg
      901 gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcagtg gtcgaatcct
      961 tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag aagaaacagg
     1021 ggatcgagga aaacctgtac ttccaatcca atattgga
//
