LOCUS       sequence-89993-e        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 High pI protein 69 TEV LIC cloning ve...
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..35
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(62..67,68..84,85..90)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(456..503)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     499..517
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(642..662)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(708..719)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattctcatg
       61 tttgacagct tatcatcgat aagctttaat gcggtagttt atcacagtta aattgctaac
      121 gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca
      181 ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata
      241 tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga
      301 tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag
      361 tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg
      421 tcctgtggat atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta
      481 gaggccccaa ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc
      541 tttcgggctt tgttagcagc cggatcagcg cgcacgcgtg gtaccggccg gccaccaggt
      601 ggcgcgccgc gatcgcggat ccgttatcca cttccaatat tggattggaa gtacaggttt
      661 tcctcgatcc cctgtttctt cttaggagcg agcaccatga tcatctggcg gccttcgatc
      721 ttcgttggga aggattcgac cactgccagt tcttgcaaat cgtctttcac gcgattaagc
      781 acttccatac cgatttgctg gtgcgccatc tcacgaccgc ggaaacgcag cgtgattttg
      841 gctttatcac cctcttcgag aaagcgaatc aggctgcgga gttttacctg atagtcgcct
      901 tcatctgtac caggacggaa tttaatttcc ttaacctgga taactttttg ctttttcttc
      961 tgttccttag aagacttgct cttttcatag aggaatttgc cgtaatccat tatacgacaa
//
