LOCUS       sequence-89999-e        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 High pI protein 68 TEV LIC cloning ve...
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(205..309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     377..395
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(422..427,428..444,445..450)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(816..863)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     859..877
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(1002..1022)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(1068..1079)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
       61 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
      121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
      181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tgttattgaa gcatttatca
      241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      301 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
      361 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattctcatg
      421 tttgacagct tatcatcgat aagctttaat gcggtagttt atcacagtta aattgctaac
      481 gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca
      541 ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata
      601 tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga
      661 tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag
      721 tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg
      781 tcctgtggat atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta
      841 gaggccccaa ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc
      901 tttcgggctt tgttagcagc cggatcagcg cgcacgcgtg gtaccggccg gccaccaggt
      961 ggcgcgccgc gatcgcggat ccgttatcca cttccaatat tggattggaa gtacaggttt
     1021 tcctcgatcc cctgtttctt cttaggagcg agcaccatga tcatctggcg gccttcgatc
     1081 ttcgttggga aggattcgac cactgccagt
//
