LOCUS       sequence-90000-e        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 High pI protein 68 TEV LIC cloning ve...
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     201..220
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     304..323
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        304..322
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             411..433
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             453..470
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             729..740
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             786..806
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(931..949)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      945..992
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
       61 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
      121 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
      181 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
      241 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
      301 aattaatacg actcactata gggagaccac aacggtttcc ctctagtgcc ggctccggag
      361 agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat tttgtttaac
      421 tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat ggttcttcta
      481 tggaacagaa gaaaaagcaa aaagttatcc aggttaagga aattaaattc cgtcctggta
      541 cagatgaagg cgactatcag gtaaaactcc gcagcctgat tcgctttctc gaagagggtg
      601 ataaagccaa aatcacgctg cgtttccgcg gtcgtgagat ggcgcaccag caaatcggta
      661 tggaagtgct taatcgcgtg aaagacgatt tgcaagaact ggcagtggtc gaatccttcc
      721 caacgaagat cgaaggccgc cagatgatca tggtgctcgc tcctaagaag aaacagggga
      781 tcgaggaaaa cctgtacttc caatccaata ttggaagtgg ataacggatc cgcgatcgcg
      841 gcgcgccacc tggtggccgg ccggtaccac gcgtgcgcgc tgatccggct gctaacaaag
      901 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      961 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc
     1021 c
//
