LOCUS       sequence-90001-e        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His10 MBP Asn10 TEV LIC cloning vector (2C...
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     201..220
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     304..323
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        304..322
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             411..433
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             453..482
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
       61 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
      121 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
      181 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
      241 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
      301 aattaatacg actcactata gggagaccac aacggtttcc ctctagtgcc ggctccggag
      361 agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat tttgtttaac
      421 tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat caccatcacc
      481 atggttcttc tatgaaaatc gaagaaggta aactggtaat ctggattaac ggcgataaag
      541 gctataacgg tctcgctgaa gtcggtaaga aattcgagaa agataccgga attaaagtca
      601 ccgttgagca tccggataaa ctggaagaga aattcccaca ggttgcggca actggcgatg
      661 gccctgacat tatcttctgg gcacacgacc gctttggtgg ctacgctcaa tctggcctgt
      721 tggctgaaat caccccggac aaagcgttcc aggacaagct gtatccgttt acctgggatg
      781 ccgtacgtta caacggcaag ctgattgctt acccgatcgc tgttgaagcg ttatcgctga
      841 tttataacaa agatctgctg ccgaacccgc caaaaacctg ggaagagatc ccggcgctgg
      901 ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa gaaccgtact
      961 tcacctggcc gctgattgct gctgacgggg gttatgcgtt caagtatgaa aacggc
//
