LOCUS       sequence-90005-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His10 MBP Asn10 TEV LIC cloning vector (2C...
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(39..86)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     82..100
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(225..245)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(303..326)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 ccgtcctgtg gatatccgga tatagttcct cctttcagca aaaaacccct caagacccgt
       61 ttagaggccc caaggggtta tgctagttat tgctcagcgg tggcagcagc caactcagct
      121 tcctttcggg ctttgttagc agccggatca gcgcgcacgc gtggtaccgg ccggccacca
      181 ggtggcgcgc cgcgatcgcg gatccgttat ccacttccaa tattggattg gaagtacagg
      241 ttttcctcga tcccgaggtt gttgttattg ttattgttgt tgttgttcga gctcgaatta
      301 gtctgcgcgt ctttcagggc ttcatcgaca gtctgacgac cgctggcggc gttgatcacc
      361 gcagtacgca cggcatacca gaaagcggac atctgcggga tgttcggcat gatttcacct
      421 ttctgggcgt tttccatagt ggcggcaata cgtggatctt tcgccaactc ttcctcgtaa
      481 gacttcagcg ctacggcacc cagcggtttg tctttattaa ccgcttccag accttcatca
      541 gtcagcagat agttttcgag gaactctttt gccagctctt tgttcggact ggcggcgtta
      601 atacctgcgc tcagcacgcc aacgaacggt ttggatggtt gacccttgaa ggtcggcagt
      661 accgttacac cataattcac tttgctggtg tcgatgttgg accatgccca cgggccgttg
      721 atggtcatcg ctgtttcgcc tttattaaag gcagcttctg cgatggagta atcggtgtct
      781 gcattcatgt gtttgttttt aatcaggtca accaggaagg tcagacccgc tttcgcgcca
      841 gcgttatcca cgcccacgtc tttaatgtcg tacttgccgt tttcatactt gaacgcataa
      901 cccccgtcag cagcaatcag cggccaggtg aagtacggtt cttgcaggtt gaacatcagc
      961 gcgctcttac
//
