LOCUS       sequence-90008-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  TITLE     TLR Signaling Plasmids
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-TLR2-YFP
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(22..735,736..738,739..741))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(67..88)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     416..435
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     675..696
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tatagaatag ggccctctag attacttgta cagctcgtcc atgccgagag tgatcccggc
       61 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
      121 ggcggactgg tagctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
      181 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
      241 cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
      301 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
      361 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
      421 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
      481 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc acttcaggcc
      541 gtagccgaag gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
      601 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
      661 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
      721 ctcctcgccc ttgctcacca tctcgaggga ctttatcgca gctctcagat ttacccaaaa
      781 tccttcccgc tgagcctcgt ccatgggcca ctccaggtag gtcttggtgt tcattatctt
      841 ccgcagcttg cagaagcgct ggggaatggc ttttttctca atgggctcca gaagaatgag
      901 aatggcagca tcattgttct catcaaaaag acggaaatgg gagaagtcca gttcatactt
      961 gcaccactca ctcttcacaa agttttcaga a
//
