LOCUS       sequence-90011-e         470 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-on) lentiviral vector for transgene (CAG 
            promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVCT-tTR-KRAB
FEATURES             Location/Qualifiers
     source          1..470
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(112..306)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             complement(409..429)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttgagctcga ctagctagct agctactagc tagtcgagat ccttaaactg atgatttgat
       61 ttcaaatgca gtctctgaat caggatgggt ctcttggtga atttctctct ccaccagcca
      121 gggctcttct cccttctcca accggaggat cacatctggc ttagtaagct gataacccaa
      181 ggaaaccagg ttcttatagt tctccagcat cacatttctg tacacgatct gctgagcagt
      241 gtccagcagc ttccactcct ccctggtgaa gtccacaaat acatccttga aggtcaccag
      301 tgtccgggac caggcagtta gtgacttagc atccatgccc tccttgttct tgatgatact
      361 tccttgagtg acagcagagt gctgaggaga caaagcacca ccgccgtcga cctttctctt
      421 cttttttggc gacccacttt cacatttaag ttgtttttct aatccgcata
//
