LOCUS       sequence-90012-e        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 GSSGSS NusA TEV LIC cloning vector (2...
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(111..131)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tcagcttcct ttcgggcttt gttagcagcc ggatcagcgc gcacgcgtgg taccggccgg
       61 ccaccaggtg gcgcgccgcg atcgcggatc cgttatccac ttccaatatt ggattggaag
      121 tacaggtttt cctcgatccc cgcttcgtca ccgaaccagc aaatgttacg ggcagccata
      181 atcagtgctc cggctttttc gtcggtcaac ccttcgatat cagccagatc atcaatgccc
      241 tgttcggcga gatcttccag cgtacaaacg ccacgggcgg ccagtttgaa tgccaaatca
      301 cgatctaccc cttcaaggtt cagcagatcg tcagccggtt tgttatcacc gaggctttct
      361 tcctgggcct gtgcaatggt ggccagtgca tttttagcac gctcgcgcag tgcttcaacg
      421 gtcggctcat caaggccttc gatttccaac agctctttca tcggcacata ggccaattct
      481 tccagcgtcg agaagccttc ttctaccaga acagtcgcga agtcttcgtc gatgtcgaga
      541 tatttggtga aggtgtcgat cgctgcgtgc gcttccgcct gatgcttagc ctgcaggtcg
      601 tcaacggtca tcacgttgag ttcccaaccg ctcagctgcg aagccagacg cacgttctga
      661 ccgttacggc caatcgcctg cgccaggtta ccggcttcaa cggcgatatc catggtgtgt
      721 ttatcttcat ccaccacgat agaagcaacg tctgccggtg ccattgcgtt aatcacgaac
      781 tgcgccgggt tatcatccca caggacgata tcgatacgct cgccacccag ttcagtagac
      841 accgcctgaa cacgcgcgcc acgcatacct acgcaagcac ctaccggatc gatacgttta
      901 tcgttggttt tcaccgcgat tttcgcacga gaacccggat cgcgagccgc tgctttaatt
      961 tcaatcactt cttcgccgat ttctggcact tcaatacgga acagttcgat cag
//
